{"doi":"10.17615/zsey-8685","title":"Regulation of membrane trafficking and organ separation by the NEVERSHED ARF-GAP protein","abstract":"Cell separation, or abscission, is a highly specialized process in plants that facilitates remodeling of their architecture and reproductive success. Because few genes are known to be essential for organ abscission, we conducted a screen for mutations that alter floral organ shedding in Arabidopsis. Nine recessive mutations that block shedding were found to disrupt the function of an ADP-ribosylation factor-GTPase-activating protein (ARF-GAP) we have named NEVERSHED (NEV). As predicted by its homology to the yeast Age2 ARF-GAP and transcriptional profile, NEV influences other aspects of plant development, including fruit growth. Co-localization experiments carried out with NEV-specific antiserum and a set of plant endomembrane markers revealed that NEV localizes to the trans-Golgi network and endosomes in Arabidopsis root epidermal cells. Interestingly, transmission electron micrographs of abscission zone regions from wild-type and nev flowers reveal defects in the structure of the Golgi apparatus and extensive accumulation of vesicles adjacent to the cell walls. Our results suggest that NEV ARF-GAP activity at the trans-Golgi network and distinct endosomal compartments is required for the proper trafficking of cargo molecules required for cell separation.","journal":"UNC Libraries","year":2021,"id":230920,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9662,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":401703,"name":"Paul A. Randazzo","orcid":"0000-0001-5349-0881","position":1,"is_corresponding":false},{"id":605365,"name":"Sarah M. Taylor","orcid":null,"position":2,"is_corresponding":false},{"id":3799,"name":"Joseph R. Ecker","orcid":"0000-0001-5799-5895","position":3,"is_corresponding":false},{"id":401697,"name":"Ruibai Luo","orcid":"0000-0002-1887-5636","position":4,"is_corresponding":false},{"id":837225,"name":"Michael W. Lewis","orcid":"0000-0002-8649-6689","position":5,"is_corresponding":false},{"id":643246,"name":"Niko Geldner","orcid":"0000-0002-2300-9644","position":6,"is_corresponding":false},{"id":28826,"name":"Joanne Chory","orcid":"0000-0002-3664-8525","position":7,"is_corresponding":false},{"id":599521,"name":"Lalitree Darnielle","orcid":null,"position":8,"is_corresponding":false},{"id":253798,"name":"Michelle E. Leslie","orcid":"0000-0003-1576-8816","position":9,"is_corresponding":false},{"id":90208,"name":"Martin F. Yanofsky","orcid":null,"position":10,"is_corresponding":false},{"id":599519,"name":"Sarah J. Liljegren","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:55:09.211909Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}