{"doi":"10.17615/z9ca-qd07","title":"Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair","abstract":"Nonhomologous end-joining (NHEJ) is the main pathway for repair of DNA double-strand breaks (DSBs), the most cytotoxic form of DNA damage resulting from ionizing radiation, chemotherapeutics, and normal cellular processes. The mechanisms that control NHEJ play key roles in development, in immunity, and in response to cancer therapy; however, the current state of knowledge regarding the physical nature of the NHEJ repair process is limited. Here we used super-resolution microscopy to define the organization of NHEJ complexes in cells, showing that long filaments form at either side of the break. Single-molecule FRET revealed dynamic behavior in which breaks can pair in an adjacent, non–end-to-end configuration.","journal":"UNC Libraries","year":2020,"id":135365,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.954,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":279107,"name":"Eli Rothenberg","orcid":"0000-0002-1382-1380","position":1,"is_corresponding":false},{"id":239779,"name":"Howard H. Chang","orcid":"0000-0002-6316-1640","position":2,"is_corresponding":false},{"id":402307,"name":"Michael R. Lieber","orcid":"0000-0003-3700-6345","position":3,"is_corresponding":false},{"id":2222,"name":"David Fenyö","orcid":"0000-0001-5049-3825","position":4,"is_corresponding":false},{"id":592104,"name":"Go Watanabe","orcid":"0000-0001-6713-1249","position":5,"is_corresponding":false},{"id":448788,"name":"Dale A. Ramsden","orcid":"0000-0003-1575-4748","position":6,"is_corresponding":false},{"id":335153,"name":"Alejandra Leo‐Macías","orcid":"0000-0002-7579-9764","position":7,"is_corresponding":false},{"id":336767,"name":"Natasha T. Strande","orcid":"0000-0001-5148-9772","position":8,"is_corresponding":false},{"id":321290,"name":"Sarah Keegan","orcid":"0000-0003-3756-7767","position":9,"is_corresponding":false},{"id":556254,"name":"Dylan A. Reid","orcid":"0000-0001-6134-1165","position":10,"is_corresponding":false},{"id":479622,"name":"Betül Akgöl Oksuz","orcid":"0000-0002-0177-4193","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:31.787520Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}