{"doi":"10.17615/z3nd-5t58","title":"Tumor Necrosis Factor Alpha-Induced Interleukin-1 Alpha Synthesis and Cell Death Is Increased in Mouse Epithelial Cells Infected With Chlamydia muridarum","abstract":"Chlamydia trachomatis-genital infection in women can be modeled in mice using Chlamydia muridarum. Using this model, it has been shown that the cytokines tumor necrosis factor (TNF)α and interleukin (IL)-1α lead to irreversible tissue damage in the oviducts. In this study, we investigated the contribution of TNFα on IL-1α synthesis in infected epithelial cells. We show that C muridarum infection enhanced TNFα-induced IL-1α expression and release in a mouse epithelial cell line. In addition to IL-1α, several TNFα-induced inflammatory genes were also highly induced, and infection enhanced TNF-induced cell death. In the mouse model of genital infection, oviducts from mice lacking the TNFα receptor displayed minimal staining for IL-1α compared with wild-type oviducts. Our results suggest TNFα and IL-1α enhance each other's downstream effects resulting in a hyperinflammatory response to chlamydial infection. We propose that biologics targeting TNF-induced IL-1α synthesis could be used to mitigate tissue damage during chlamydial infection.","journal":"UNC Libraries","year":2024,"id":501476,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9562,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":229818,"name":"Erin Harrell","orcid":null,"position":1,"is_corresponding":false},{"id":784150,"name":"Ian Huntress","orcid":null,"position":2,"is_corresponding":false},{"id":793594,"name":"Clare E. Gyorke","orcid":null,"position":3,"is_corresponding":false},{"id":228252,"name":"Hayden Brochu","orcid":"0000-0002-7234-3235","position":4,"is_corresponding":false},{"id":497307,"name":"Uma M. Nagarajan","orcid":"0000-0003-2164-9338","position":5,"is_corresponding":false},{"id":529239,"name":"Shanmugam Nagarajan","orcid":null,"position":6,"is_corresponding":false},{"id":798851,"name":"Crescentia Cho","orcid":"0000-0003-4753-802X","position":7,"is_corresponding":false},{"id":228260,"name":"Xinxia Peng","orcid":"0000-0002-0117-3582","position":8,"is_corresponding":false},{"id":269877,"name":"J. Ashley Ezzell","orcid":"0000-0002-5142-4685","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:10:15.999370Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}