{"doi":"10.17615/yzya-ay28","title":"Poly-acetylated chromatin signatures are preferred epitopes for site-specific histone H4 acetyl antibodies","abstract":"Antibodies specific for histone post-translational modifications (PTMs) have been central to our understanding of chromatin biology. Here, we describe an unexpected and novel property of histone H4 site-specific acetyl antibodies in that they prefer poly-acetylated histone substrates. By all current criteria, these antibodies have passed specificity standards. However, we find these site-specific histone antibodies preferentially recognize chromatin signatures containing two or more adjacent acetylated lysines. Significantly, we find that the poly-acetylated epitopes these antibodies prefer are evolutionarily conserved and are present at levels that compete for these antibodies over the intended individual acetylation sites. This alarming property of acetyl-specific antibodies has far-reaching implications for data interpretation and may present a challenge for the future study of acetylated histone and non-histone proteins.","journal":"UNC Libraries","year":2020,"id":142346,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":275708,"name":"Krzysztof Krajewski","orcid":"0000-0001-7159-617X","position":1,"is_corresponding":false},{"id":282215,"name":"Scott B. Rothbart","orcid":"0000-0003-1631-5392","position":2,"is_corresponding":false},{"id":71449,"name":"Brian D. Strahl","orcid":"0000-0002-4947-6259","position":3,"is_corresponding":false},{"id":608909,"name":"Lin Shu","orcid":"0000-0001-7468-8766","position":4,"is_corresponding":false},{"id":237417,"name":"Michael‐Christopher Keogh","orcid":"0000-0002-2219-8623","position":5,"is_corresponding":false},{"id":227837,"name":"Benjamin A. García","orcid":"0000-0002-2306-1207","position":6,"is_corresponding":false},{"id":609399,"name":"Laura-Mae Britton","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:31.424593Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}