{"doi":"10.17615/ykc5-7b39","title":"Comparison of Two Human Immunodeficiency Virus (HIV) RNA Surrogate Assays to the Standard HIV RNA Assay","abstract":"Human immunodeficiency virus (HIV) RNA testing is the gold standard for monitoring antiretroviral therapy in HIV-infected patients. However, equipment and reagent costs preclude widespread use of the assay in resource-limited settings. The Perkin-Elmer Ultrasensitive p24 assay and the Cavidi Exavir Load assay both offer potentially simpler, less costly technologies for monitoring viral load. These assays were compared to the Roche Amplicor HIV-1 Monitor Test, v1.5, using panels of clinical samples (subtype B) from HIV-positive subjects and HIV-spiked samples (subtypes A, C, D, CRF_01AE, CRF_02AG, and F). The Ultrasensitive p24 assay detected 100% of the spiked samples with virus loads of >250,000copies/ml and 61% of the clinical samples with virus loads of 219 to 288,850 copies/ml. Detection rates were improved substantially if an external lysis buffer was added to the procedure. The Cavidi assay detected 54 to 100% of spiked samples with virus loads >10,000 copies/ml and 68% of the clinical samples. These detection rates were also greatly improved with a newly implemented version of this kit. Coefficients of variation demonstrate good reproducibility for each of these kits. The results from the Cavidi v1.0, Cavidi v2.0, and Perkin-Elmer, and the Perkin-Elmer Plus external buffers all correlated well with the results from the Roche Monitor Test (r = 0.83 to 0.96, r = 0.84 to 0.99, r = 0.58 to 0.67, and r = 0.59 to 0.95, respectively). Thus, the use of these two assays for monitoring patients, together with less-frequent confirmation testing, offers a feasible alternative to frequent HIV RNA testing in resource-limited settings.","journal":"UNC Libraries","year":2020,"id":114913,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":4,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9539,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":324055,"name":"Donald Brambilla","orcid":"0000-0002-7276-4689","position":1,"is_corresponding":false},{"id":540432,"name":"R. Respess","orcid":null,"position":2,"is_corresponding":false},{"id":121269,"name":"W. Stevens","orcid":null,"position":3,"is_corresponding":false},{"id":540433,"name":"S. Scianna","orcid":null,"position":4,"is_corresponding":false},{"id":540434,"name":"A. Cachafeiro","orcid":null,"position":5,"is_corresponding":false},{"id":540435,"name":"J. Schupbach","orcid":null,"position":6,"is_corresponding":false},{"id":435191,"name":"S. A. Fiscus","orcid":null,"position":7,"is_corresponding":false},{"id":257182,"name":"C. Jennings","orcid":null,"position":8,"is_corresponding":false},{"id":540436,"name":"R. J. Morack","orcid":null,"position":9,"is_corresponding":false},{"id":540437,"name":"J. Simon Gronowitz","orcid":null,"position":10,"is_corresponding":false},{"id":540438,"name":"A. D. Danilovic","orcid":null,"position":11,"is_corresponding":false},{"id":540439,"name":"M. A. Ussery","orcid":null,"position":12,"is_corresponding":false},{"id":540440,"name":"J. W. Bremer","orcid":null,"position":13,"is_corresponding":false},{"id":540441,"name":"G. E. Corrigan","orcid":null,"position":14,"is_corresponding":false},{"id":540442,"name":"O. E. Varnier","orcid":null,"position":15,"is_corresponding":false},{"id":540054,"name":"S. M. Crowe","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:13:33.226937Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}