{"doi":"10.17615/xqer-hc05","title":"Divergent Synthesis of Heparan Sulfate Oligosaccharides","abstract":"Heparan sulfates are implicated in a wide range of biological processes. A major challenge in deciphering their structure and activity relationship is the synthetic difficulties to access diverse heparan sulfate oligosaccharides with well-defined sulfation patterns. In order to expedite the synthesis, a divergent synthetic strategy was developed. By integrating chemical synthesis and two types of O-sulfo transferases, seven different hexasaccharides were obtained from a single hexasaccharide precursor. This approach combined the flexibility of chemical synthesis with the selectivity of enzyme-catalyzed sulfations, thus simplifying the overall synthetic operations. In an attempt to establish structure activity relationships of heparan sulfate binding with its receptor, the synthesized oligosaccharides were incorporated onto a glycan microarray, and their bindings with a growth factor FGF-2 were examined. The unique combination of chemical and enzymatic approaches expanded the capability of oligosaccharide synthesis. In addition, the well-defined heparan sulfate structures helped shine light on the fine substrate specificities of biosynthetic enzymes and confirm the potential sequence of enzymatic reactions in biosynthesis.","journal":"Figshare","year":2020,"id":138786,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9521,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":600850,"name":"Bo Yang","orcid":"0000-0003-4210-8864","position":1,"is_corresponding":false},{"id":308597,"name":"Xuefei Huang","orcid":"0000-0002-6468-5526","position":2,"is_corresponding":false},{"id":288975,"name":"Yongmei Xu","orcid":"0000-0002-3756-581X","position":3,"is_corresponding":false},{"id":270756,"name":"Peng Wang","orcid":"0009-0009-0391-7379","position":4,"is_corresponding":false},{"id":288986,"name":"Jian Liu","orcid":"0000-0001-8552-1400","position":5,"is_corresponding":false},{"id":595517,"name":"Steven B. Dulaney","orcid":null,"position":6,"is_corresponding":false},{"id":593343,"name":"Zhen Wang","orcid":"0000-0001-7188-5828","position":7,"is_corresponding":false},{"id":600851,"name":"Gopinath Tiruchinapally","orcid":"0000-0001-8393-894X","position":8,"is_corresponding":false},{"id":601410,"name":"J. Kathawa","orcid":null,"position":9,"is_corresponding":false},{"id":600849,"name":"Mohammad H. El‐Dakdouki","orcid":"0000-0003-0679-2267","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:17:06.651725Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}