{"doi":"10.17615/vsmc-y237","title":"Airway stem cell reconstitution by the transplantation of primary or pluripotent stem cell-derived basal cells","abstract":"Life-long reconstitution of a tissue's resident stem cell compartment with engrafted cells has the potential to durably replenish organ function. Here, we demonstrate the engraftment of the airway epithelial stem cell compartment via intra-airway transplantation of mouse or human primary and pluripotent stem cell (PSC)-derived airway basal cells (BCs). Murine primary or PSC-derived BCs transplanted into polidocanol-injured syngeneic recipients give rise for at least two years to progeny that stably display the morphologic, molecular, and functional phenotypes of airway epithelia. The engrafted basal-like cells retain extensive self-renewal potential, evident by the capacity to reconstitute the tracheal epithelium through seven generations of secondary transplantation. Using the same approach, human primary or PSC-derived BCs transplanted into NOD scid gamma (NSG) recipient mice similarly display multilineage airway epithelial differentiation in vivo. Our results may provide a step toward potential future syngeneic cell-based therapy for patients with diseases resulting from airway epithelial cell damage or dysfunction.","journal":"UNC Libraries","year":2024,"id":502494,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9509,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":240008,"name":"Andrew Berical","orcid":"0000-0002-0818-0259","position":1,"is_corresponding":false},{"id":266489,"name":"Xaralabos Varelas","orcid":"0000-0002-2882-4541","position":2,"is_corresponding":false},{"id":240009,"name":"Taylor Matte","orcid":"0000-0002-7796-7485","position":3,"is_corresponding":false},{"id":228731,"name":"Darrell N. Kotton","orcid":"0000-0002-9604-8476","position":4,"is_corresponding":false},{"id":228728,"name":"Michael J. Herriges","orcid":"0000-0002-7842-9515","position":5,"is_corresponding":false},{"id":1352394,"name":"Aimee D. Kohn","orcid":null,"position":6,"is_corresponding":false},{"id":967693,"name":"Fan Wang","orcid":"0000-0002-9869-9504","position":7,"is_corresponding":false},{"id":534231,"name":"F. Hawkins","orcid":null,"position":8,"is_corresponding":false},{"id":36215,"name":"Hiroaki Kiyokawa","orcid":"0000-0002-7942-6455","position":9,"is_corresponding":false},{"id":1345800,"name":"M.L Beermann","orcid":null,"position":10,"is_corresponding":false},{"id":550792,"name":"Liang Ma","orcid":"0000-0003-3524-6695","position":11,"is_corresponding":false},{"id":1352395,"name":"B R Thapa","orcid":null,"position":12,"is_corresponding":false},{"id":266488,"name":"Andrew Tilston-Lünel","orcid":"0000-0002-5582-6884","position":13,"is_corresponding":false},{"id":228729,"name":"Alexandra B. Ysasi","orcid":"0000-0002-2932-870X","position":14,"is_corresponding":false},{"id":260367,"name":"Pushpinder Bawa","orcid":"0000-0002-8370-3638","position":15,"is_corresponding":false},{"id":365692,"name":"S.H. Randell","orcid":null,"position":16,"is_corresponding":false},{"id":868130,"name":"Jake A. Le Suer","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-19T02:10:23.709105Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}