{"doi":"10.17615/vp0r-8e16","title":"Human Myo19 Is a Novel Myosin that Associates with Mitochondria","abstract":"Mitochondria are pleomorphic organelles [1, 2] that have central roles in cell physiology. Defects in their localization and dynamics lead to human disease [3-5]. Myosins are actin-based motors that power processes such as muscle contraction, cytokinesis, and organelle transport [6]. Here we report the initial characterization of myosin-XIX (Myo19), the founding member of a novel class of myosin that associates with mitochondria. The 970aa heavy chain consists of a motor domain, three IQ motifs, and a short tail. Myo19 mRNA is expressed in multiple tissues and antibodies to human Myo19 detect a ∼109kD band in multiple cell lines. Both endogenous Myo19 and GFP-Myo19 exhibit striking localization to mitochondria. Deletion analysis reveals that the Myo19 tail is necessary and sufficient for mitochondrial localization. Expressing full-length GFP-Myo19 in A549 cells reveals a remarkable gain-of-function where the majority of the mitochondria move continuously. Moving mitochondria travel for many microns with an obvious leading end and distorted shape. The motility and shape-change are sensitive to latrunculin B, indicating that both are actin-dependent. Expressing the GFP-Myo19 tail in CAD cells resulted in decreased mitochondrial run lengths in neurites. These results suggest that this novel myosin functions as an actin-based motor for mitochondrial movement in vertebrate cells.","journal":"UNC Libraries","year":2020,"id":141932,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9649,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":608439,"name":"Marianela Feliu","orcid":null,"position":1,"is_corresponding":false},{"id":608440,"name":"Melisa B. Kortan","orcid":null,"position":2,"is_corresponding":false},{"id":596318,"name":"Lindsay B. Case","orcid":"0000-0003-3166-1138","position":3,"is_corresponding":false},{"id":308269,"name":"Rebecca C. Adikes","orcid":"0000-0002-7526-8701","position":4,"is_corresponding":false},{"id":361939,"name":"Michelle Rengarajan","orcid":"0000-0003-3203-7986","position":5,"is_corresponding":false},{"id":607927,"name":"Audun Lier","orcid":"0000-0001-7548-4140","position":6,"is_corresponding":false},{"id":275052,"name":"Richard E. Cheney","orcid":"0000-0001-6565-7888","position":7,"is_corresponding":false},{"id":608441,"name":"Niki S. Panaretos","orcid":null,"position":8,"is_corresponding":false},{"id":608442,"name":"Stephanie Q. Slater","orcid":null,"position":9,"is_corresponding":false},{"id":608443,"name":"Melinda M. DiVito","orcid":null,"position":10,"is_corresponding":false},{"id":598627,"name":"Omar A. Quintero","orcid":"0000-0002-9314-1704","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:27.861239Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}