{"doi":"10.17615/vjg3-b325","title":"Gβ Association and Effector Interaction Selectivities of the Divergent Gγ Subunit Gγ 13","abstract":"G gamma(13) is a divergent member of the G gamma subunit family considered to be a component of the gustducin G-protein heterotrimer involved in bitter and sweet taste reception in taste bud cells. G gamma(13) contains a C-terminal asparagine-proline-tryptophan (NPW) tripeptide, a hallmark of RGS protein G gamma-like (GGL) domains which dimerize exclusively with G beta(5) subunits. In this study, we investigated the functional range of G gamma(13) assembly with G beta subunits using multiple assays of G beta association and G beta gamma effector modulation. G gamma(13) was observed to associate with all five G beta subunits (G beta(1-5)) upon co-translation in vitro, as well as function with all five G beta subunits in the modulation of Kir3.1/3.4 (GIRK1/4) potassium and N-type (alpha(1B)) calcium channels. Multiple G beta/G gamma(13) pairings were also functional in cellular assays of phospholipase C (PLC) beta 2 activation and inhibition of G alpha(q)-stimulated PLC beta 1 activity. However, upon cellular co-expression of G gamma(13) with different G beta subunits, only G beta(1)/G gamma(13), G beta(3)/G gamma(13), and G beta(4)/G gamma(13) pairings were found to form stable dimers detectable by co-immunoprecipitation under high-detergent cell lysis conditions. Collectively, these data indicate that G gamma(13) forms functional G beta gamma dimers with a range of G beta subunits. Coupled with our detection of G gamma(13) mRNA in mouse and human brain and retina, these results imply that this divergent G gamma subunit can act in signal transduction pathways other than that dedicated to taste reception in sensory lingual tissue.","journal":"UNC Libraries","year":2021,"id":231123,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9579,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":602142,"name":"Douglas A. Bayliss","orcid":"0000-0002-5630-2572","position":1,"is_corresponding":false},{"id":838331,"name":"Qiubo Lei","orcid":null,"position":2,"is_corresponding":false},{"id":836738,"name":"Rebecca A. Morris","orcid":null,"position":3,"is_corresponding":false},{"id":838332,"name":"Jennie R. Hillmann","orcid":null,"position":4,"is_corresponding":false},{"id":400242,"name":"Richard J. Miller","orcid":"0000-0002-8254-6714","position":5,"is_corresponding":false},{"id":838333,"name":"Janice Y. Zhou","orcid":null,"position":6,"is_corresponding":false},{"id":836128,"name":"Michele R. Wing","orcid":"0000-0001-9688-0396","position":7,"is_corresponding":false},{"id":601373,"name":"Bonita L. Blake","orcid":null,"position":8,"is_corresponding":false},{"id":515775,"name":"T. Kendall Harden","orcid":null,"position":9,"is_corresponding":false},{"id":836737,"name":"Cynthia I. Behe","orcid":null,"position":10,"is_corresponding":false},{"id":437994,"name":"David P. Siderovski","orcid":"0000-0002-0688-8210","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:55:12.400773Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}