{"doi":"10.17615/vfe3-bf32","title":"Covalent Modifications of Histone H3K9 Promote Binding of CHD3","abstract":"Chromatin remodeling is required for genome function and is facilitated by ATP-dependent complexes, such as nucleosome remodeling and deacetylase (NuRD). Among its core components is the chromodomain helicase DNA binding protein 3 (CHD3) whose functional significance is not well established. Here, we show that CHD3 co-localizes with the other NuRD subunits, including HDAC1, near the H3K9ac-enriched promoters of the NuRD target genes. The tandem PHD fingers of CHD3 bind histone H3 tails and posttranslational modifications that increase hydrophobicity of H3K9-methylation or acetylation (H3K9me3 or H3K9ac)-enhance this interaction. Binding of CHD3 PHDs promotes H3K9Cme3-nucleosome unwrapping in vitro and perturbs the pericentric heterochromatin structure in vivo. Methylation or acetylation of H3K9 uniquely alleviates the intra-nucleosomal interaction of histone H3 tails, increasing H3K9 accessibility. Collectively, our data suggest that the targeting of covalently modified H3K9 by CHD3 might be essential in diverse functions of NuRD.","journal":"UNC Libraries","year":2020,"id":137661,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9524,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":145895,"name":"Adam H. Tencer","orcid":null,"position":1,"is_corresponding":false},{"id":329213,"name":"Yi Zhang","orcid":"0000-0001-8170-9983","position":2,"is_corresponding":false},{"id":598316,"name":"Joseph B. Bridgers","orcid":"0000-0002-0585-4131","position":3,"is_corresponding":false},{"id":71449,"name":"Brian D. Strahl","orcid":"0000-0002-4947-6259","position":4,"is_corresponding":false},{"id":598317,"name":"Hidetoshi Kono","orcid":"0000-0001-5729-8707","position":5,"is_corresponding":false},{"id":598893,"name":"Jennifer K. Sims","orcid":null,"position":6,"is_corresponding":false},{"id":598318,"name":"Jinzen Ikebe","orcid":"0000-0003-3692-6879","position":7,"is_corresponding":false},{"id":598894,"name":"Michael A. Darcy","orcid":null,"position":8,"is_corresponding":false},{"id":598319,"name":"Wei Li","orcid":"0000-0002-8449-3839","position":9,"is_corresponding":false},{"id":111546,"name":"Michael G. Poirier","orcid":"0000-0002-1563-5792","position":10,"is_corresponding":false},{"id":37098,"name":"Jeffrey J. Hayes","orcid":"0000-0001-6981-1803","position":11,"is_corresponding":false},{"id":247177,"name":"Paul A. Wade","orcid":"0000-0002-6042-357X","position":12,"is_corresponding":false},{"id":598895,"name":"Hillary F. Allen","orcid":null,"position":13,"is_corresponding":false},{"id":598320,"name":"Di Luo","orcid":"0000-0003-0124-7518","position":14,"is_corresponding":false},{"id":398196,"name":"Xiaodong Wang","orcid":"0000-0002-4304-5482","position":15,"is_corresponding":false},{"id":314067,"name":"Tatiana G. Kutateladze","orcid":"0000-0001-7375-6990","position":16,"is_corresponding":false},{"id":480147,"name":"Khan L. Cox","orcid":null,"position":17,"is_corresponding":false},{"id":311911,"name":"Jie Lyu","orcid":"0000-0002-6530-5257","position":18,"is_corresponding":false},{"id":598321,"name":"Matthew D. Gibson","orcid":"0000-0001-5424-0585","position":19,"is_corresponding":false},{"id":598322,"name":"Tyler Weaver","orcid":"0000-0002-5138-7140","position":20,"is_corresponding":false},{"id":398007,"name":"Catherine A. Musselman","orcid":"0000-0002-8356-7971","position":21,"is_corresponding":false},{"id":240953,"name":"Jovylyn Gatchalian","orcid":"0000-0003-0322-4619","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:59.449905Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}