{"doi":"10.17615/t50r-r277","title":"Direct Thy-1/αVβ3 integrin interaction mediates neuron to astrocyte communication","abstract":"Thy-1 is an abundant neuronal glycoprotein of poorly defined function. We recently provided evidence indicating that Thy-1 clusters a β3-containing integrin in astrocytes to induce tyrosine phosphorylation, RhoA activation and the formation of focal adhesions and stress fibers. To date, the α subunit partner of β3 integrin in DI TNC1 astrocytes is unknown. Similarly, the ability of neuronal, membrane-bound Thy-1 to trigger astrocyte signaling via integrin engagement remains speculation. Here, evidence that αv forms an αvβ3 heterodimer in DI TNC1 astrocytes was obtained. In neuron-astrocyte association assays, the presence of either anti-αv or anti-β3 integrin antibodies reduced cell-cell interaction demonstrating the requirement of both integrin subunits for this association. Moreover, anti-Thy-1 antibodies blocked stimulation of astrocytes by neurons but not the binding of these two cell types. Thus, neuron-astrocyte association involved binding between molecular components in addition to the Thy-1-integrin; however, the signaling events leading to focal adhesion formation in astrocytes depended exclusively on the latter interaction. Additionally, wild-type (RLD) but not mutated (RLE) Thy-1 was shown to directly interact with αvβ3 integrin by Surface Plasmon Resonance analysis. This interaction was promoted by divalent cations and was species-independent. Together, these results demonstrate that the αvβ3 integrin heterodimer interacts directly with Thy-1 present on neuronal cells to stimulate astrocytes.","journal":"UNC Libraries","year":2020,"id":136586,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.952,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":595014,"name":"Sang-Uk Nham","orcid":"0000-0001-7854-759X","position":1,"is_corresponding":false},{"id":497633,"name":"Pascal Schneider","orcid":"0000-0003-0677-9409","position":2,"is_corresponding":false},{"id":341957,"name":"Andrew F. G. Quest","orcid":"0000-0001-8755-1590","position":3,"is_corresponding":false},{"id":497635,"name":"Lisette Leyton","orcid":"0000-0001-9690-9638","position":4,"is_corresponding":false},{"id":595015,"name":"Daniel Muñoz","orcid":"0009-0001-8816-8283","position":5,"is_corresponding":false},{"id":595737,"name":"Tamara Hermosilla","orcid":null,"position":6,"is_corresponding":false},{"id":595016,"name":"Nicolás Muñoz","orcid":"0000-0003-1690-447X","position":7,"is_corresponding":false},{"id":595017,"name":"Rodrigo Herrera‐Molina","orcid":"0000-0001-9597-5131","position":8,"is_corresponding":false},{"id":497629,"name":"Alejandra Valdivia","orcid":"0000-0003-1492-5066","position":9,"is_corresponding":false},{"id":392676,"name":"Keith Burridge","orcid":"0000-0002-2152-9670","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:48.793683Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}