{"doi":"10.17615/stpz-j523","title":"A PDZ-Binding Motif Controls Basolateral Targeting of Syndecan-1 Along the Biosynthetic Pathway in Polarized Epithelial Cells","abstract":"The cell surface proteoglycan, syndecan-1, is essential for normal epithelial morphology and function. Syndecan-1 is selectively localized to the basolateral domain of polarized epithelial cells and interacts with cytosolic PDZ (PSD-95, discs large, ZO-1) domain-containing proteins. Here, we show that the polarity of syndecan-1 is determined by its type II PDZ-binding motif. Mutations within the PDZ-binding motif lead to the mislocalization of syndecan-1 to the apical surface. In contrast to previous examples, however, PDZ-binding motif-dependent polarity is not determined by retention at the basolateral surface but rather by polarized sorting prior to syndecan-1’s arrival at the plasma membrane. Although none of the four known PDZ-binding partners of syndecan-1 appears to control basolateral localization, our results show that the PDZ-binding motif of syndecan-1 is decoded along the biosynthetic pathway establishing a potential role for PDZ-mediated interactions in polarized sorting.","journal":"UNC Libraries","year":2020,"id":135364,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9529,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":592098,"name":"Heike Fölsch","orcid":"0000-0003-0897-0311","position":1,"is_corresponding":false},{"id":592099,"name":"James M. Anderson","orcid":"0000-0002-8079-4044","position":2,"is_corresponding":false},{"id":592788,"name":"Jeff Sfakianos","orcid":null,"position":3,"is_corresponding":false},{"id":558745,"name":"Zenta Walther","orcid":"0000-0003-2878-361X","position":4,"is_corresponding":false},{"id":592100,"name":"Eric Anderson","orcid":"0000-0002-2601-4385","position":5,"is_corresponding":false},{"id":592101,"name":"Sandra Maday","orcid":"0000-0001-8664-4592","position":6,"is_corresponding":false},{"id":592102,"name":"Henry C. Chang","orcid":"0000-0002-4939-0463","position":7,"is_corresponding":false},{"id":592103,"name":"Ayano Satoh","orcid":"0000-0003-3736-1283","position":8,"is_corresponding":false},{"id":268287,"name":"James Shorter","orcid":"0000-0001-5269-8533","position":9,"is_corresponding":false},{"id":227918,"name":"Ira Mellman","orcid":"0000-0002-6132-7299","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:31.787520Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}