{"doi":"10.17615/skea-s522","title":"TGF-β Type II Receptor/MCP-5 Axis: At the Crossroad between Joint and Growth Plate Development","abstract":"Despite its clinical significance, the mechanisms of joint morphogenesis are still elusive. Here, we show by combining laser-capture microdissection for RNA sampling with microarray analysis, that the setting in which joint-forming interzone cells develop is distinct from adjacent growth plate chondrocytes and is characterized by down-regulation of chemokines, such as monocyte-chemoattractant protein-5 (MCP-5). Using in-vivo, ex-vivo and in-vitro approaches, we showed that low levels of interzone-MCP-5 are essential for joint formation and contribute to proper growth plate organization. Mice lacking the TGF-β-type-II-receptor (TβRII) in their limbs (Tgfbr2Prx1KO), which lack joint development and fail chondrocyte hypertrophy, showed up-regulation of interzone-MCP-5. In-vivo and ex-vivo blockade of the sole MCP-5 receptor, CCR2, in Tgfbr2Prx1KO led to rescue of joint formation and growth plate maturation; while in control mice determined an acceleration of endochondral growth plate mineralization. Taken together, we characterized the TβRII/MCP-5 axis as an essential crossroad for joint development and endochondral growth.","journal":"UNC Libraries","year":2020,"id":138957,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9572,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":598853,"name":"Clara Contaldo","orcid":null,"position":1,"is_corresponding":false},{"id":598286,"name":"Hüseyin Özkan","orcid":"0000-0001-5753-8985","position":2,"is_corresponding":false},{"id":286424,"name":"Tieshi Li","orcid":"0000-0002-7656-1854","position":3,"is_corresponding":false},{"id":598287,"name":"Lara Longobardi","orcid":"0000-0002-0156-847X","position":4,"is_corresponding":false},{"id":524972,"name":"Anna Spagnoli","orcid":"0000-0002-4497-7150","position":5,"is_corresponding":false},{"id":546740,"name":"Timothy J. Myers","orcid":"0000-0003-2502-2989","position":6,"is_corresponding":false},{"id":601027,"name":"Ying Li","orcid":"0000-0002-4286-876X","position":7,"is_corresponding":false},{"id":601686,"name":"Lynda O’Rear","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:06.651725Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}