{"doi":"10.17615/sd96-5j61","title":"Combined Atomic Force Microscope and Volumetric Light Sheet System for Correlative Force and Fluorescence Mechanobiology Studies","abstract":"The central goals of mechanobiology are to understand how cells generate force and how they respond to environmental mechanical stimuli. A full picture of these processes requires high-resolution, volumetric imaging with time-correlated force measurements. Here we present an instrument that combines an open-top, single-objective light sheet fluorescence microscope with an atomic force microscope (AFM), providing simultaneous volumetric imaging with high spatiotemporal resolution and high dynamic range force capability (10 pN – 100 nN). With this system we have captured lysosome trafficking, vimentin nuclear caging, and actin dynamics on the order of one second per single-cell volume. To showcase the unique advantages of combining Line Bessel light sheet imaging with AFM, we measured the forces exerted by a macrophage during FcɣR-mediated phagocytosis while performing both sequential two-color, fixed plane and volumetric imaging of F-actin. This unique instrument allows for a myriad of novel studies investigating the coupling of cellular dynamics and mechanical forces.","journal":"UNC Libraries","year":2023,"id":403503,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9499,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":149022,"name":"T. Watanabe","orcid":null,"position":1,"is_corresponding":false},{"id":8921,"name":"S. Grinstein","orcid":"0000-0002-6460-8694","position":2,"is_corresponding":false},{"id":1156926,"name":"M.R. Falvo","orcid":null,"position":3,"is_corresponding":false},{"id":599001,"name":"K.M. Hahn","orcid":null,"position":4,"is_corresponding":false},{"id":1156934,"name":"M.E. Kern","orcid":null,"position":5,"is_corresponding":false},{"id":1181292,"name":"B.M. Condon","orcid":null,"position":6,"is_corresponding":false},{"id":1181293,"name":"E.T. O’Brien","orcid":null,"position":7,"is_corresponding":false},{"id":1181294,"name":"M. Boyce","orcid":null,"position":8,"is_corresponding":false},{"id":1181295,"name":"J.P. Hsiao","orcid":null,"position":9,"is_corresponding":false},{"id":244547,"name":"Richard Superfine","orcid":"0000-0002-2569-071X","position":10,"is_corresponding":false},{"id":1181296,"name":"E. Nelsen","orcid":null,"position":11,"is_corresponding":false},{"id":1181289,"name":"C.M. Hobson","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T01:20:36.280647Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}