{"doi":"10.17615/pngh-d206","title":"Reconstitution of long and short patch mismatch repair reactions using Saccharomyces cerevisiae proteins","abstract":"This study demonstrates mismatch repair (MMR) reactions reconstituted in vitro with purified Saccharomyces cerevisiae proteins. Biochemical analysis of MMR in vitro showed that MMR required mispair binding by the MutS homolog 2–MutS homolog 6 complex and corresponded to the Exonuclease 1-dependent subpathway of MMR. The reactions observed involved the formation of long excision tracts whose length was consistent with the length of MMR-dependent gene conversion tracts in vivo. The availability of this reconstituted MMR reaction now allows the wealth of mutations affecting MMR generated from the genetic analysis of S. cerevisiae MMR mechanisms in vivo to be used in biochemical reconstitution studies whose ultimate goal is to reconstitute MMR linked to both DNA replication and recombination.","journal":"UNC Libraries","year":2020,"id":113869,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9534,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":537253,"name":"N. Bowen","orcid":null,"position":1,"is_corresponding":false},{"id":537254,"name":"S. Willcox","orcid":null,"position":2,"is_corresponding":false},{"id":537255,"name":"Anushka Srivatsan","orcid":null,"position":3,"is_corresponding":false},{"id":537256,"name":"R. D. Kolodner","orcid":null,"position":4,"is_corresponding":false},{"id":537257,"name":"C. E. Smith","orcid":null,"position":5,"is_corresponding":false},{"id":510735,"name":"J. D. Griffith","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:13:25.964732Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}