{"doi":"10.17615/nd6a-cs86","title":"A potent anti-dengue human antibody preferentially recognizes the conformation of E protein monomers assembled on the virus surface","abstract":"Dengue virus (DENV), which consists of four serotypes (DENV1-4), infects over 400million people annually. Previous studies have indicated most human monoclonal antibodies (HMAbs) from dengue patients are cross-reactive and poorly neutralizing. Rare neutralizing HMAbs are usually serotype-specific and bind to quaternary structure-dependent epitopes. We determined the structure of DENV1 complexed with Fab fragments of a highly potent HMAb 1F4 to 6Å resolution by cryo-EM. Although HMAb 1F4 appeared to bind to virus and not E proteins in ELISAs in the previous study, our structure showed that the epitope is located within an envelope (E) protein monomer, and not across neighboring E proteins. The Fab molecules bind to domain I (DI), and DI-DII hinge of the E protein. We also showed that HMAb 1F4 can neutralize DENV at different stages of viral entry in a cell type and receptor dependent manner. The structure reveals the mechanism by which this potent and specific antibody blocks viral infection.Subject Categories Microbiology, Virology & Host Pathogen Interaction; Immunology","journal":"UNC Libraries","year":2020,"id":74168,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":12,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9694,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":236674,"name":"Eva Harris","orcid":"0000-0002-7238-4037","position":1,"is_corresponding":false},{"id":303772,"name":"Shee‐Mei Lok","orcid":"0000-0003-4631-8041","position":2,"is_corresponding":false},{"id":106531,"name":"James E. Crowe","orcid":"0000-0002-0049-1079","position":3,"is_corresponding":false},{"id":388360,"name":"Scott A. Smith","orcid":"0000-0002-2143-2342","position":4,"is_corresponding":false},{"id":303768,"name":"G. Fibriansah","orcid":"0000-0002-9990-3335","position":5,"is_corresponding":false},{"id":105519,"name":"Aravinda de Silva","orcid":null,"position":6,"is_corresponding":false},{"id":388361,"name":"Jiaqi Wang","orcid":"0009-0005-4951-0970","position":7,"is_corresponding":false},{"id":303767,"name":"Thiam‐Seng Ng","orcid":"0000-0002-1668-8976","position":8,"is_corresponding":false},{"id":389485,"name":"Joanne L. Tan","orcid":null,"position":9,"is_corresponding":false},{"id":303769,"name":"V.A. Kostyuchenko","orcid":"0000-0001-9751-307X","position":10,"is_corresponding":false},{"id":389486,"name":"Kristie D Ibarra","orcid":null,"position":11,"is_corresponding":false},{"id":389484,"name":"Adamberage R de Alwis","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T21:45:52.171053Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}