{"doi":"10.17615/mqd8-7g42","title":"Radiation Combined With Thermal Injury Induces Immature Myeloid Cells","abstract":"The continued development of nuclear weapons and the potential for thermonuclear injury necessitates the further understanding of the immune consequences after radiation combined with injury (RCI). We hypothesized that sub-lethal ionization radiation exposure combined with a full thickness thermal injury would result in the production of immature myeloid cells. Mice underwent either a 20% total body surface area (TBSA) full-thickness contact burn or sham procedure followed by a single whole body dose of 5-Gy radiation. Serum, spleen and peripheral lymph nodes were harvested at 3 and 14 days post-injury. Flow cytometry was performed to identify and characterize adaptive and innate cell compartments. Elevated pro- and anti-inflammatory serum cytokines and profound leukopenia were observed after RCI. A population of cells with dual expression of the cell surface markers Gr-1 and CD11b were identified in all experimental groups, but was significantly elevated after burn alone and RCI at 14 days post-injury. In contrast to the T-cell suppressive nature of myeloid-derived suppressor cells (MDSC) found after trauma and sepsis, myeloid cells after RCI augmented T-cell proliferation and were associated with a weak but significant increase in IFN-γ and a decrease in IL-10. This is consistent with previous work in burn injury indicating that a MDSC-like population increases innate immunity. RCI results in the increase of distinct populations of Gr-1+ CD11b+cells within the secondary lymphoid organs, and we propose these immature inflammatory myeloid cells provide innate immunity to the severely injured and immunocompromised host.","journal":"UNC Libraries","year":2020,"id":139005,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9679,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":601097,"name":"Amal Khoury","orcid":"0000-0002-6501-7167","position":1,"is_corresponding":false},{"id":324101,"name":"Anthony Charles","orcid":"0000-0001-7332-4354","position":2,"is_corresponding":false},{"id":324099,"name":"Bruce A. Cairns","orcid":"0000-0003-2360-2840","position":3,"is_corresponding":false},{"id":225651,"name":"Gregory D. Sempowski","orcid":"0000-0003-0391-6594","position":4,"is_corresponding":false},{"id":601098,"name":"April E. Mendoza","orcid":"0000-0002-9773-4515","position":5,"is_corresponding":false},{"id":527806,"name":"Laurel B. Kartchner","orcid":null,"position":6,"is_corresponding":false},{"id":225654,"name":"Jenny P.‐Y. Ting","orcid":"0000-0002-3282-419X","position":7,"is_corresponding":false},{"id":225644,"name":"W. June Brickey","orcid":"0000-0002-6503-539X","position":8,"is_corresponding":false},{"id":551791,"name":"Robert Maile","orcid":"0000-0002-9126-4188","position":9,"is_corresponding":false},{"id":601771,"name":"Crystal J. Neely","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:06.651725Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}