{"doi":"10.17615/mk5c-0c09","title":"Development and Validation of Multiplex Assays for Mouse and Human IgG and IgA to Neisseria gonorrhoeae Antigens","abstract":"There is an urgent need for vaccines against Neisseria gonorrhoeae (Ng), the causative agent of gonorrhea. Vaccination with an outer-membrane vesicle (OMV)-based Neisseria meningitidis (Nm) vaccine provides some protection from Ng; however, the mechanisms underlying this cross-protection are unknown. To address this need, we developed multiplexed bead-based assays for the relative quantification of human and mouse IgG and IgA against Ng antigens. The assays were evaluated for analyte independence, dilutional linearity, specificity, sensitivity, intra- and inter-assay variability, and robustness to sample storage conditions. The assay was then used to test samples from mice and humans immunized with an Nm-OMV vaccine.","journal":"UNC Libraries","year":2025,"id":580565,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9582,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":619687,"name":"Erica L. Stover","orcid":null,"position":1,"is_corresponding":false},{"id":1081127,"name":"Lixin Li","orcid":"0000-0003-2821-7108","position":2,"is_corresponding":false},{"id":339442,"name":"Robert A. Nicholas","orcid":"0000-0003-2507-8974","position":3,"is_corresponding":false},{"id":394340,"name":"Marcia M. Hobbs","orcid":null,"position":4,"is_corresponding":false},{"id":277691,"name":"Joseph A. Duncan","orcid":"0000-0002-4438-2018","position":5,"is_corresponding":false},{"id":277694,"name":"Ann E. Jerse","orcid":"0000-0002-5118-3172","position":6,"is_corresponding":false},{"id":277687,"name":"Kristie L. Connolly","orcid":"0000-0002-7516-4427","position":7,"is_corresponding":false},{"id":225650,"name":"Andrew N. Macintyre","orcid":"0000-0002-5297-0207","position":8,"is_corresponding":false},{"id":574997,"name":"Aleksandra E. Sikora","orcid":"0000-0001-7977-2596","position":9,"is_corresponding":false},{"id":277692,"name":"Marguerite B. Little","orcid":"0000-0002-9796-9162","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:58:43.046112Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}