{"doi":"10.17615/man9-xc95","title":"ANCA patients have T cells responsive to complementary PR-3 antigen","abstract":"Some patients with proteinase 3 specific anti-neutrophil cytoplasmic autoantibodies (PR3-ANCA) also have antibodies that react to complementary-PR3 (cPR3), a protein encoded by the antisense RNA of the PR3 gene. To study whether patients with anti-cPR3 antibodies have cPR3-responsive memory T cells we selected conditions that allowed cultivation of memory cells but not naïve cells. About half of the patients were found to have CD4+TH1 memory cells responsive to the cPR3138-169-peptide; while only a third of the patients had HI-PR3 protein responsive T cells. A significant number of T cells from patients responded to cPR3138-169 peptide and to HI-PR3 protein by proliferation and/or secretion of IFN-γ, compared to healthy controls while there was no response to scrambled peptide. Cells responsive to cPR3138-169-peptide were not detected in MPO-ANCA patients suggesting that this response is specific. The HLADRB1* 15 allele was significantly overrepresented in our patient group and is predicted to bind cPR3138-169 peptide with high affinity. Regression analysis showed a significant likelihood that anti-cPR3 antibodies and cPR3-specific T cells coexist in individuals, consistent with an immunological history of encounter with a PR3-complementary protein. We suggest that the presence of cells reacting to potential complementary protein pairs might provide an alternative mechanism for auto-immune diseases.","journal":"UNC Libraries","year":2020,"id":112735,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9602,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":533173,"name":"Barrak M. Pressler","orcid":"0000-0003-1253-5937","position":1,"is_corresponding":false},{"id":533174,"name":"Jiajin Yang","orcid":"0009-0006-8978-1318","position":2,"is_corresponding":false},{"id":533175,"name":"David J. Bautz","orcid":"0000-0003-0053-1250","position":3,"is_corresponding":false},{"id":533962,"name":"Gloria A. Preston","orcid":null,"position":4,"is_corresponding":false},{"id":533963,"name":"Lionaki, Sofia","orcid":null,"position":5,"is_corresponding":false},{"id":533176,"name":"Roland Tisch","orcid":"0000-0001-7751-7241","position":6,"is_corresponding":false},{"id":492235,"name":"John L. Schmitz","orcid":"0000-0002-1425-236X","position":7,"is_corresponding":false},{"id":480783,"name":"Susan L. Hogan","orcid":"0000-0001-7205-8357","position":8,"is_corresponding":false},{"id":533964,"name":"Hyunsook Chin","orcid":null,"position":9,"is_corresponding":false},{"id":25195,"name":"Ronald J. Falk","orcid":"0000-0003-2274-4847","position":10,"is_corresponding":false},{"id":25194,"name":"J. Charles Jennette","orcid":"0000-0002-8081-6565","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:13:17.845093Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}