{"doi":"10.17615/kc0h-2g09","title":"SARS-CoV-2 RBD trimer protein adjuvanted with Alum-3M-052 protects from SARS-CoV-2 infection and immune pathology in the lung","abstract":"There is a great need for the development of vaccines that induce potent and long-lasting protective immunity against SARS-CoV-2. Multimeric display of the antigen combined with potent adjuvant can enhance the potency and longevity of the antibody response. The receptor binding domain (RBD) of the spike protein is a primary target of neutralizing antibodies. Here, we developed a trimeric form of the RBD and show that it induces a potent neutralizing antibody response against live virus with diverse effector functions and provides protection against SARS-CoV-2 challenge in mice and rhesus macaques. The trimeric form induces higher neutralizing antibody titer compared to monomer with as low as 1μg antigen dose. In mice, adjuvanting the protein with a TLR7/8 agonist formulation alum-3M-052 induces 100-fold higher neutralizing antibody titer and superior protection from infection compared to alum. SARS-CoV-2 infection causes significant loss of innate cells and pathology in the lung, and vaccination protects from changes in innate cells and lung pathology. These results demonstrate RBD trimer protein as a suitable candidate for vaccine against SARS-CoV-2.","journal":"UNC Libraries","year":2021,"id":229846,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9544,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":834936,"name":"K. Floyd","orcid":null,"position":1,"is_corresponding":false},{"id":590913,"name":"K.H. Dinnon","orcid":null,"position":2,"is_corresponding":false},{"id":834937,"name":"Patrick Shi","orcid":null,"position":3,"is_corresponding":false},{"id":834938,"name":"L. Lai","orcid":null,"position":4,"is_corresponding":false},{"id":834939,"name":"Seung-A Shin","orcid":null,"position":5,"is_corresponding":false},{"id":834940,"name":"Ayalew Negash Shiferaw","orcid":null,"position":6,"is_corresponding":false},{"id":834895,"name":"M. Tomai","orcid":null,"position":7,"is_corresponding":false},{"id":834941,"name":"R.R. Amara","orcid":null,"position":8,"is_corresponding":false},{"id":834942,"name":"T.H. Vanderford","orcid":null,"position":9,"is_corresponding":false},{"id":834943,"name":"N. Cheedarla","orcid":null,"position":10,"is_corresponding":false},{"id":801371,"name":"M.S. Suthar","orcid":null,"position":11,"is_corresponding":false},{"id":319338,"name":"S. Fischinger","orcid":null,"position":12,"is_corresponding":false},{"id":834888,"name":"C.B. Fox","orcid":null,"position":13,"is_corresponding":false},{"id":834944,"name":"V.V. Edara","orcid":null,"position":14,"is_corresponding":false},{"id":319363,"name":"C. Atyeo","orcid":null,"position":15,"is_corresponding":false},{"id":834945,"name":"N.K. Routhu","orcid":null,"position":16,"is_corresponding":false},{"id":834946,"name":"S. Gangadhara","orcid":null,"position":17,"is_corresponding":false},{"id":834947,"name":"S. Kirejczyk","orcid":null,"position":18,"is_corresponding":false},{"id":391460,"name":"L. Gralinski","orcid":null,"position":19,"is_corresponding":false},{"id":834948,"name":"Ashish Sahoo","orcid":null,"position":20,"is_corresponding":false},{"id":834949,"name":"V.D. Menachery","orcid":null,"position":21,"is_corresponding":false},{"id":834950,"name":"T.M. Styles","orcid":null,"position":22,"is_corresponding":false},{"id":319347,"name":"G. Alter","orcid":null,"position":23,"is_corresponding":false},{"id":834951,"name":"V.S. Bollimpelli","orcid":null,"position":24,"is_corresponding":false},{"id":801370,"name":"S. Gumber","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:55:01.675482Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}