{"doi":"10.17615/ff5h-b937","title":"Characterizing optical coherence tomography speckle fluctuation spectra of mammary organoids during suppression of intracellular motility","abstract":"Background: An understanding of how the mammary gland responds to toxicant and drug exposures can shed light on mechanisms of breast cancer initiation/progression and therapeutic effectiveness, respectively. In this study, we employed noninvasive, label-free and high-throughput optical coherence tomography speckle fluctuation spectroscopy (OCT-SFS) to track exposure-response relationships in three-dimensional (3D) mammary epithelial organoid models. Methods: OCT-SFS is sensitive to relatively high speed (~0.16-8 μm/min) motions of subcellular light scattering components occurring over short (~2-114 s) time scales, termed \"intracellular motility.\" In this study, OCT speckle fluctuation spectra are quantified by two metrics: the intracellular motility amplitude, M, and frequency-dependent motility roll-off, α. OCT-SFS was performed on human mammary organoid models comprised of pre-malignant MCF10DCIS.com cells or MCF7 adenocarcinoma cells over 6 days of exposure to either a microtubule inhibitor (Paclitaxel, Taxol) or a myosin II inhibitor (Blebbistatin). Raw values of a and M were normalized to a dynamic range corresponding to fixed (0%) and live/homeostatic (100%) organoids for each cell line. Results: In this work, we observed a significant decrease in both M and a of MCF10DCIS.com organoids after 24 hours of exposure to Taxol (P<0.001), and a significant decrease only in α for MCF7 organoids after 48 hours of exposure (P<0.0001). We also observed a significant decrease in both M and α of MCF7 organoids at the longest exposure time of 6 days to Blebbistatin (P<0.0001), and a significant decrease only in M for MCF10DCIS.com organoids after 24 hours of exposure (P<0.01). Conclusions: OCT-SFS revealed cell line-specific response patterns, in terms of intracellular motility, to different motility suppression mechanisms. This provides a foundation for future OCT-SFS studies of longitudinal responses of the mammary gland in toxicology and drug research.","journal":"UNC Libraries","year":2023,"id":383379,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":2,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9496,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":270126,"name":"Melissa A. Troester","orcid":"0000-0001-9506-6624","position":1,"is_corresponding":false},{"id":1150193,"name":"L. Yang","orcid":"0000-0002-8868-5977","position":2,"is_corresponding":false},{"id":1148657,"name":"A.M. Fuller","orcid":null,"position":3,"is_corresponding":false},{"id":530625,"name":"Amy L. Oldenburg","orcid":"0000-0002-2642-8350","position":4,"is_corresponding":false},{"id":1150192,"name":"Xin Yu","orcid":"0000-0002-8690-4275","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T01:17:25.158896Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}