{"doi":"10.17615/eerq-4123","title":"Genetic determination of vitamin D status in Collaborative Cross mice","abstract":"Vitamin D is an essential micronutrient that plays many important roles in the body. Deficiency of vitamin D has been associated with various diseases, including rickets in children, osteomalacia in adults, type 1 and type 2 diabetes. Vitamin D status is affected by both environmental and genetic factors. However, the mechanisms of genetic determination are unclear. In this project, we aim to characterize the genetic determinants of vitamin D status using Collaborative Cross (CC) inbred mice. Genetic differences at 14 genes important for vitamin D metabolism were examined across 16 CC mice. This was achieved by analyzing single nucleotide polymorphisms (SNPs) at the 14 genes using principal component analysis (PCA). Levels of 4 vitamin D metabolites (25(OH)D3, 1,25(OH)2D3, 24,25(OH)2D3 and 1,24,25(OH)3D3) in 4 CC strains (CC001, CC006, CC011 and CC026) were measured and compared across strains. The genetic differences determined from PCA analyses were then associated to basal levels of vitamin D metabolites as well as the response dietary depletion. Basal levels of 25(OH)D3 and 24,25(OH)2D3 were significantly (adj.p<0.5) different among the 4 CC strains. Genes Cyp27b1, Vdr, Ets1, Trim35 were significantly (adj.p<0.5) associated with basal level of 24,25(OH)2D3. In conclusions, this project showed that CC mice are a valuable model to study genetic determination of vitamin D status, due the their genetic diversity and wide range of vitamin D status. We also demonstrated that genetic differences among CC strains determined from PCA analyses can be used to predict their basal level of 24,25(OH)2D3.","journal":"UNC Libraries","year":2021,"id":224411,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9493,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":826832,"name":"Changran Niu","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:54:18.470602Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}