{"doi":"10.17615/e67z-nh56","title":"Reply to Troth et al","abstract":"We thank Troth et al. for the opportunity to extend the discussion of our data on the mutagenicity of N4-hydroxycytidine (rNHC) [1]. We view our work as providing the proof-of-concept showing that as rNHC is phosphorylated to its active ribonucleoside 5’-triphosphate, the ribonucleoside 5’-diphosphate intermediate that is the immediate precursor to the ribonucleoside 5’-triphosphate also plays the equivalent role of an intermediate precursor for the synthesis of 2’-deoxyribonucleoside 5’-diphosphate (by the activity of ribonucleotide reductase). This is the normal pathway for the synthesis of DNA precursors used from bacteria to humans; thus, it should not be a question of whether the mutagenic form of dNHC as a precursor to DNA is formed, but rather what the impact is. On this point we have unpublished cell-based data supporting conversion of rNHC to dNHC, albeit at low intracellular levels. Also, the near identity of rNHC to cytidine (the addition of a single oxygen atom) makes it likely that rNHC and cytidine undergo similar metabolism in the cell.","journal":"UNC Libraries","year":2021,"id":229821,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9568,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":552475,"name":"Collin S Hill","orcid":"0000-0003-3968-1862","position":1,"is_corresponding":false},{"id":416700,"name":"Shuntai Zhou","orcid":"0000-0002-8353-386X","position":2,"is_corresponding":false},{"id":247475,"name":"Ronald Swanstrom","orcid":"0000-0001-7777-0773","position":3,"is_corresponding":false},{"id":362853,"name":"Blaide M D Woodburn","orcid":null,"position":4,"is_corresponding":false},{"id":228075,"name":"Raymond F. Schinazi","orcid":"0000-0002-6688-3614","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:55:01.675482Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}