{"doi":"10.17615/e5ns-se69","title":"Crystal Structure of the Plant Epigenetic Protein Arginine Methyltransferase 10","abstract":"Protein arginine methyltransferase 10 (PRMT10) is a type-I arginine methyltransferase essential for regulating flowering time in Arabidopsis thaliana (At). We present a 2.6 Å resolution crystal structure of AtPRMT10 in complex with a reaction product, S-adenosylhomocysteine. The structure reveals a dimerization arm 12–20 residues longer than PRMT structures elucidated previously; as a result, the essential AtPRMT10 dimer exhibits a large central cavity and a distinctly accessible active site. We employ molecular dynamics to examine how dimerization facilitates AtPRMT10 motions necessary for activity, and show that these motions are conserved in other PRMT enzymes. Finally, functional data reveal that the N-terminal ten residues of AtPRMT10 influence substrate specificity, and that enzyme activity is dependent on substrate protein sequences distal from the methylation site. Taken together, these data provide insights into the molecular mechanism of Arabidopsis thaliana PRMT10 as well as other members of the PRMT family of enzymes. They highlight differences between AtPRMT10 and other PRMTs, but also indicate that motions are a conserved element of PRMT function.","journal":"UNC Libraries","year":2020,"id":136131,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9219,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":36347,"name":"Matthew R. Redinbo","orcid":"0000-0003-0814-5346","position":1,"is_corresponding":false},{"id":56208,"name":"Xiaofeng Cao","orcid":"0000-0001-9871-0753","position":2,"is_corresponding":false},{"id":595070,"name":"Monica Frazier","orcid":null,"position":3,"is_corresponding":false},{"id":594532,"name":"Falong Lu","orcid":"0000-0002-8321-4818","position":4,"is_corresponding":false},{"id":594531,"name":"Yuan Cheng","orcid":"0000-0003-1830-7951","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:48.793683Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}