{"doi":"10.17615/dz8z-st19","title":"Engineering extrinsic disorder to control protein activity in living cells","abstract":"Optogenetic and chemogenetic control of proteins has revealed otherwise inaccessible facets of signaling dynamics. Here we use light or ligand-sensitive domains to modulate the structural disorder of diverse proteins, thereby generating robust allosteric switches. Sensory domains were inserted into non-conserved, surface exposed loops that were tight and identified computationally as allosterically coupled to active sites. Allosteric switches introduced into motility signaling proteins (kinases, GTPases, guanine exchange factors) controlled conversion between conformations closely resembling natural active and inactive states, and modulated the morphodynamics of living cells. Our results illustrate a broadly applicable approach to design physiological protein switches.","journal":"UNC Libraries","year":2020,"id":137531,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9546,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":282904,"name":"Ilme Schlichting","orcid":"0000-0002-0936-7496","position":1,"is_corresponding":false},{"id":410303,"name":"Pei‐Hsuan Chu","orcid":null,"position":2,"is_corresponding":false},{"id":163454,"name":"Miroslaw Tarnawski","orcid":"0000-0001-5297-5668","position":3,"is_corresponding":false},{"id":237740,"name":"Klaus M. Hahn","orcid":"0000-0002-1970-7562","position":4,"is_corresponding":false},{"id":149320,"name":"Nikolay V. Dokholyan","orcid":"0000-0002-8225-4025","position":5,"is_corresponding":false},{"id":592704,"name":"David Shirvanyants","orcid":null,"position":6,"is_corresponding":false},{"id":548436,"name":"Onur Dağliyan","orcid":"0000-0003-1825-1011","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:56.001887Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}