{"doi":"10.17615/dxdb-w057","title":"Chemical shift imprint of intersubunit communication in a symmetric homodimer","abstract":"Regulatory signals between protein subunits depend on communication between sequential binding events. How such long-range communication, or allostery, operates at the subdomain level has been elusive, especially for homooligomeric proteins. To address this problem, homodimers of thymidylate synthase were generated for optimized study of individual protomers of the singly bound dimer. Mixed 15N-labeled dimers were created with a single functional active site, allowing site-specific, protomer-specific chemical shifts to report on step-wise binding effects. Long-range intersubunit communication was observed although this communication was apparent only in the second ligand-binding step, in which changes were in the first ligand-bound region. Visualization of up to four peaks for each residue amide provides a unique way to assess the allosteric mechanism.","journal":"UNC Libraries","year":2020,"id":136195,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9565,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":594598,"name":"Bradley T. Falk","orcid":"0009-0006-6009-3765","position":1,"is_corresponding":false},{"id":547166,"name":"Paul J. Sapienza","orcid":"0000-0001-5279-3653","position":2,"is_corresponding":false},{"id":149321,"name":"Andrew L. Lee","orcid":"0000-0003-2783-1907","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:48.793683Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}