{"doi":"10.17615/dj89-pm49","title":"A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation of Behavior","abstract":"DREADDs are chemogenetic tools widely used to remotely control cellular signaling, neuronal activity and behavior. Here we used a structure-based approach to develop a new Gi coupled DREADD using the kappa-opioid receptor as template (KORD) that is activated by the pharmacologically inert ligand salvinorin B (SALB). Activation of virally-expressed KORD in several neuronal contexts robustly attenuated neuronal activity and modified behaviors. Additionally, co-expression of the KORD and the Gq coupled M3-DREADD within the same neuronal population facilitated the sequential and bi-directional remote control of behavior. The availability of DREADDs activated by different ligands provides enhanced opportunities for investigating diverse physiological systems using multiplexed chemogenetic actuators.","journal":"UNC Libraries","year":2020,"id":117616,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":1,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.95,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":444598,"name":"Eyal Vardy","orcid":null,"position":1,"is_corresponding":false},{"id":268293,"name":"Daniel J. Urban","orcid":"0000-0002-2672-8468","position":2,"is_corresponding":false},{"id":548260,"name":"Patrick M. Giguère","orcid":"0000-0002-7520-8290","position":3,"is_corresponding":false},{"id":330587,"name":"Joseph E. Rittiner","orcid":"0000-0003-3592-6500","position":4,"is_corresponding":false},{"id":512312,"name":"Juan Song","orcid":"0000-0001-5872-6806","position":5,"is_corresponding":false},{"id":397283,"name":"Nicole A. Crowley","orcid":"0000-0002-1142-7929","position":6,"is_corresponding":false},{"id":103670,"name":"Xi‐Ping Huang","orcid":"0000-0002-2585-653X","position":7,"is_corresponding":false},{"id":548261,"name":"Kate White","orcid":"0000-0002-1439-0228","position":8,"is_corresponding":false},{"id":548262,"name":"Philip D. Mosier","orcid":"0000-0003-0964-3806","position":9,"is_corresponding":false},{"id":257078,"name":"Hu Zhu","orcid":null,"position":10,"is_corresponding":false},{"id":375893,"name":"Kristen E. Pleil","orcid":"0000-0001-6300-9414","position":11,"is_corresponding":false},{"id":225467,"name":"Jeffrey F. DiBerto","orcid":"0000-0002-8227-2071","position":12,"is_corresponding":false},{"id":248901,"name":"Chia Li","orcid":null,"position":13,"is_corresponding":false},{"id":248899,"name":"Christopher M. Mazzone","orcid":null,"position":14,"is_corresponding":false},{"id":249826,"name":"Thomas L. Kash","orcid":"0000-0002-4747-4495","position":15,"is_corresponding":false},{"id":548853,"name":"Flori M. Sassano","orcid":null,"position":16,"is_corresponding":false},{"id":225466,"name":"Reid H. J. Olsen","orcid":"0000-0003-4621-949X","position":17,"is_corresponding":false},{"id":247794,"name":"Michael J. Krashes","orcid":"0000-0003-0966-3401","position":18,"is_corresponding":false},{"id":303872,"name":"J. Elliott Robinson","orcid":"0000-0001-9417-3938","position":19,"is_corresponding":false},{"id":544665,"name":"C. J. Malanga","orcid":null,"position":20,"is_corresponding":false},{"id":225473,"name":"Bryan L. Roth","orcid":"0000-0002-0561-6520","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:13:54.951170Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}