{"doi":"10.17615/dgx1-p829","title":"Stabilized Coronavirus Spike Stem Elicits a Broadly Protective Antibody","abstract":"Current coronavirus vaccines primarily target immunodominant epitopes in the S1 subunit, which are poorly conserved and susceptible to escape mutations, thus threatening vaccine efficacy. Here, we use structure-guided protein engineering to remove the S1 subunit from the MERS-CoV spike (S) glycoprotein and develop stabilized stem (SS) antigens. Vaccination with MERS SS elicits cross-reactive β-coronavirus antibody responses and protects mice against lethal MERS-CoV challenge. High-throughput screening of antibody secreting cells from MERS SS-immunized mice leads to discovery of a panel of cross-reactive monoclonal antibodies. Among them, antibody IgG22 binds with high affinity to both MERS-CoV and SARS-CoV-2 S proteins, and a combination of electron microscopy and crystal structures localizes the epitope to a conserved coiled-coil region in the S2 subunit. Passive transfer of IgG22 protects mice against both MERS-CoV and SARS-CoV-2 challenge. Collectively, these results provide proof-of-principle for cross-reactive coronavirus antibodies and inform the development of pan-coronavirus vaccines and therapeutic antibodies.","journal":"UNC Libraries","year":2021,"id":229845,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.948,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":5946,"name":"Ethan J. Fritch","orcid":"0000-0003-1096-8958","position":1,"is_corresponding":false},{"id":87308,"name":"Jory A. Goldsmith","orcid":"0000-0003-2917-0620","position":2,"is_corresponding":false},{"id":5952,"name":"Ralph S. Baric","orcid":"0000-0001-6827-8701","position":3,"is_corresponding":false},{"id":105924,"name":"Chia‐Wei Chou","orcid":"0000-0001-9618-338X","position":4,"is_corresponding":false},{"id":266887,"name":"Anne P. Werner","orcid":"0000-0003-2322-6830","position":5,"is_corresponding":false},{"id":105602,"name":"Ande West","orcid":"0000-0001-7381-9533","position":6,"is_corresponding":false},{"id":87312,"name":"Jason S. McLellan","orcid":"0000-0003-3991-542X","position":7,"is_corresponding":false},{"id":615972,"name":"Krithika Muthuraman","orcid":"0000-0003-0361-772X","position":8,"is_corresponding":false},{"id":105500,"name":"Kenneth H. Dinnon","orcid":"0000-0002-8942-1551","position":9,"is_corresponding":false},{"id":105611,"name":"James D. Chappell","orcid":"0000-0002-7382-7864","position":10,"is_corresponding":false},{"id":87310,"name":"Olubukola M. Abiona","orcid":"0000-0002-2745-1691","position":11,"is_corresponding":false},{"id":105111,"name":"Sarah R. Leist","orcid":"0000-0002-4989-5381","position":12,"is_corresponding":false},{"id":615976,"name":"Ester Falconer","orcid":"0000-0003-1486-5251","position":13,"is_corresponding":false},{"id":105126,"name":"Mark R. Denison","orcid":"0000-0003-2655-0900","position":14,"is_corresponding":false},{"id":105604,"name":"Laura J. Stevens","orcid":"0000-0003-4386-6958","position":15,"is_corresponding":false},{"id":615958,"name":"Kathryn Westendorf","orcid":"0000-0002-7354-9470","position":16,"is_corresponding":false},{"id":762061,"name":"Ching‐Lin Hsieh","orcid":"0000-0001-9468-9969","position":17,"is_corresponding":false},{"id":87307,"name":"Kizzmekia S. Corbett","orcid":"0000-0002-2641-4688","position":18,"is_corresponding":false},{"id":87311,"name":"Barney S. Graham","orcid":"0000-0001-8112-0853","position":19,"is_corresponding":false},{"id":590473,"name":"Alexandra Schäfer","orcid":"0000-0002-4760-4923","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:55:01.675482Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}