{"doi":"10.17615/cv2y-n773","title":"Nanoparticle display of prefusion coronavirus spike elicits S1-focused cross-reactive antibody response against diverse coronavirus subgenera","abstract":"Multivalent antigen display is a fast-growing area of interest toward broadly protective vaccines. Current nanoparticle-based vaccine candidates demonstrate the ability to confer antibody-mediated immunity against divergent strains of notably mutable viruses. In coronaviruses, this work is predominantly aimed at targeting conserved epitopes of the receptor binding domain. However, targeting conserved non-RBD epitopes could limit the potential for antigenic escape. To explore new potential targets, we engineered protein nanoparticles displaying coronavirus prefusion-stabilized spike (CoV_S-2P) trimers derived from MERS-CoV, SARS-CoV-1, SARS-CoV-2, hCoV-HKU1, and hCoV-OC43 and assessed their immunogenicity in female mice. Monotypic SARS-1 nanoparticles elicit cross-neutralizing antibodies against MERS-CoV and protect against MERS-CoV challenge. MERS and SARS nanoparticles elicit S1-focused antibodies, revealing a conserved site on the S N-terminal domain. Moreover, mosaic nanoparticles co-displaying distinct CoV_S-2P trimers elicit antibody responses to distant cross-group antigens and protect male and female mice against MERS-CoV challenge. Our findings will inform further efforts toward the development of pan-coronavirus vaccines.","journal":"UNC Libraries","year":2023,"id":413698,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9525,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2023-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1044814,"name":"Charis Palandjian","orcid":null,"position":1,"is_corresponding":false},{"id":1195249,"name":"Hutchinson G.B.","orcid":null,"position":2,"is_corresponding":false},{"id":1195250,"name":"C S","orcid":null,"position":3,"is_corresponding":false},{"id":1195251,"name":"Ziwawo C.T.","orcid":null,"position":4,"is_corresponding":false},{"id":332064,"name":"N. Wang","orcid":"0009-0002-2192-5566","position":5,"is_corresponding":false},{"id":1195252,"name":"Abiona O.M.","orcid":null,"position":6,"is_corresponding":false},{"id":278043,"name":"Dan Ellis","orcid":"0000-0003-3410-7503","position":7,"is_corresponding":false},{"id":249511,"name":"Masaru Kanekiyo","orcid":"0000-0001-5767-1532","position":8,"is_corresponding":false},{"id":1195253,"name":"Fritch E.J","orcid":null,"position":9,"is_corresponding":false},{"id":1195254,"name":"Werner A.P.","orcid":null,"position":10,"is_corresponding":false},{"id":1195255,"name":"King N.P.","orcid":null,"position":11,"is_corresponding":false},{"id":105706,"name":"David Baker","orcid":"0000-0001-7896-6217","position":12,"is_corresponding":false},{"id":105570,"name":"Seyhan Boyoglu-Barnum","orcid":null,"position":13,"is_corresponding":false},{"id":105602,"name":"Ande West","orcid":"0000-0001-7381-9533","position":14,"is_corresponding":false},{"id":1193608,"name":"Leist S.R","orcid":null,"position":15,"is_corresponding":false},{"id":249503,"name":"George Ueda","orcid":"0000-0002-9792-7149","position":16,"is_corresponding":false},{"id":87305,"name":"Daniel Wrapp","orcid":"0000-0002-0538-9647","position":17,"is_corresponding":false},{"id":229148,"name":"Yaroslav Tsybovsky","orcid":"0000-0002-2159-5248","position":18,"is_corresponding":false},{"id":1185287,"name":"Baric R.S","orcid":null,"position":19,"is_corresponding":false},{"id":1195256,"name":"Graham B.S.","orcid":null,"position":20,"is_corresponding":false},{"id":1195248,"name":"McLellan J.S.","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T01:21:57.641539Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}