{"doi":"10.17615/ckx5-4h52","title":"Global metabolic analyses identify key differences in metabolite levels between polymyxin-susceptible and polymyxin-resistant Acinetobacter baumannii","abstract":"Multidrug-resistant Acinetobacter baumannii presents a global medical crisis and polymyxins are used as the last-line therapy. This study aimed to identify metabolic differences between polymyxin-susceptible and polymyxin-resistant A. baumannii using untargeted metabolomics. The metabolome of each A. baumannii strain was measured using liquid chromatography-mass spectrometry. Multivariate and univariate statistics and pathway analyses were employed to elucidate metabolic differences between the polymyxin-susceptible and -resistant A. baumannii strains. Significant differences were identified between the metabolic profiles of the polymyxin-susceptible and -resistant A. baumannii strains. The lipopolysaccharide (LPS) deficient, polymyxin-resistant 19606R showed perturbation in specific amino acid and carbohydrate metabolites, particularly pentose phosphate pathway (PPP) and tricarboxylic acid (TCA) cycle intermediates. Levels of nucleotides were lower in the LPS-deficient 19606R. Furthermore, 19606R exhibited a shift in its glycerophospholipid profile towards increased abundance of short-chain lipids compared to the parent polymyxin-susceptible ATCC 19606. In contrast, in a pair of clinical isolates 03–149.1 (polymyxin-susceptible) and 03–149.2 (polymyxin-resistant, due to modification of lipid A), minor metabolic differences were identified. Notably, peptidoglycan biosynthesis metabolites were significantly depleted in both of the aforementioned polymyxin-resistant strains. This is the first comparative untargeted metabolomics study to show substantial differences in the metabolic profiles of the polymyxin-susceptible and -resistant A. baumannii.","journal":"UNC Libraries","year":2020,"id":136295,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9508,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":304456,"name":"Darren J. Creek","orcid":"0000-0001-7497-7082","position":1,"is_corresponding":false},{"id":258542,"name":"Jiangning Song","orcid":"0000-0001-8031-9086","position":2,"is_corresponding":false},{"id":258541,"name":"Jian Li","orcid":"0000-0001-7953-8230","position":3,"is_corresponding":false},{"id":287873,"name":"Anthony W. Purcell","orcid":"0000-0003-0532-8331","position":4,"is_corresponding":false},{"id":285698,"name":"Matthew D. Johnson","orcid":"0000-0002-0808-3336","position":5,"is_corresponding":false},{"id":361335,"name":"John D. Boyce","orcid":"0000-0002-8614-3074","position":6,"is_corresponding":false},{"id":532664,"name":"Soon-Ee Cheah","orcid":null,"position":7,"is_corresponding":false},{"id":595343,"name":"Mei-Ling Han","orcid":null,"position":8,"is_corresponding":false},{"id":594707,"name":"Mohd Hafidz Mahamad Maifiah","orcid":"0000-0002-3165-0343","position":9,"is_corresponding":false},{"id":350710,"name":"Alan Forrest","orcid":"0000-0003-2752-4819","position":10,"is_corresponding":false},{"id":232748,"name":"Keith S. Kaye","orcid":"0000-0003-2051-1732","position":11,"is_corresponding":false},{"id":506148,"name":"Visanu Thamlikitkul","orcid":"0000-0002-0044-5988","position":12,"is_corresponding":false},{"id":350102,"name":"Paul J. Hertzog","orcid":"0000-0002-1373-8472","position":13,"is_corresponding":false},{"id":326951,"name":"Tony Velkov","orcid":"0000-0002-0017-7952","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:48.793683Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}