{"doi":"10.17615/cbwz-pm13","title":"The NLR Adaptor ASC/PYCARD Regulates DUSP10, Mitogen-activated Protein Kinase (MAPK), and Chemokine Induction Independent of the Inflammasome","abstract":"ASC/PYCARD is a common adaptor for a diverse set of inflammasomes that activate caspase-1, most prominently the NLR-based inflammasome. Mounting evidence indicates that ASC and these NLRs also elicit non-overlapping functions, but the molecular basis for this difference is unclear. To address this, we performed microarray and network analysis of ASC shRNA knockdown cells. In pathogen-infected cells, an ASC-dependent interactome is centered on the mitogen-activated protein kinase (MAPK) ERK and on multiple chemokines. ASC did not affect the expression of MAPK but affected its phosphorylation by pathogens and Toll-like receptor agonists via suppression of the dual-specificity phosphatase, DUSP10/MKP5. Chemokine induction, DUSP function, and MAPK phosphorylation were independent of caspase-1 and IL-1β. MAPK activation by pathogen was abrogated in Asc−/− but not Nlrp3−/−, Nlrc4−/−, or Casp1−/− macrophages. These results demonstrate a function for ASC that is distinct from the inflammasome in modulating MAPK activity and chemokine expression and further identify DUSP10 as a novel ASC target.","journal":"UNC Libraries","year":2020,"id":142004,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9574,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":502184,"name":"Debra J. Taxman","orcid":null,"position":1,"is_corresponding":false},{"id":393695,"name":"Max Tze-Han Huang","orcid":null,"position":2,"is_corresponding":false},{"id":608538,"name":"Elizabeth Holley-Guthrie","orcid":null,"position":3,"is_corresponding":false},{"id":392048,"name":"Denis Gris","orcid":"0000-0003-1215-2434","position":4,"is_corresponding":false},{"id":297448,"name":"Irving C. Allen","orcid":"0000-0001-9573-5250","position":5,"is_corresponding":false},{"id":538817,"name":"Jenny Pan Yun Ting","orcid":null,"position":6,"is_corresponding":false},{"id":599740,"name":"Chris B. Moore","orcid":"0000-0003-0957-0440","position":7,"is_corresponding":false},{"id":109672,"name":"Yu L. Lei","orcid":"0000-0002-9868-9824","position":8,"is_corresponding":false},{"id":438011,"name":"Dan T. Bergstralh","orcid":"0000-0003-1689-3715","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:27.861239Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}