{"doi":"10.17615/a74k-x982","title":"Acetylation Negatively Regulates Glycogen Phosphorylase by Recruiting Protein Phosphatase 1","abstract":"Glycogen phosphorylase (GP) catalyzes the rate-limiting step in glycogen catabolism and plays a key role in maintaining cellular and organismal glucose homeostasis. GP is the first protein whose function was discovered to be regulated by reversible protein phosphorylation, which is controlled by phosphorylase kinase (PhK) and protein phosphatase 1 (PP1). Here, we report that lysine acetylation negatively regulates GP activity by both inhibiting enzyme activity directly and promoting dephosphorylation. Acetylation of GP Lys470 enhances its interaction with the PP1 substrate targeting subunit, GL, and PP1, thereby promoting GP dephosphorylation and inactivation. We show that GP acetylation is stimulated by glucose and insulin and inhibited by glucagon. Our results provide molecular insights into the intricate regulation of the classical GP and a functional cross-talk between protein acetylation and phosphorylation.","journal":"UNC Libraries","year":2020,"id":110840,"datarank":0.29188652235829704,"base_score":1.9459101490553132,"endowment":1.9459101490553132,"self_citation_contribution":0.29188652235829704,"citation_network_contribution":0.0,"self_endowment_contribution":0.29188652235829704,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":6,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9585,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":526982,"name":"Wei Xu","orcid":"0009-0006-3042-608X","position":1,"is_corresponding":false},{"id":399574,"name":"Shiwen Wang","orcid":"0000-0001-6294-4865","position":2,"is_corresponding":false},{"id":228830,"name":"Shimin Zhao","orcid":"0000-0002-3674-9107","position":3,"is_corresponding":false},{"id":255072,"name":"Dan Ye","orcid":"0000-0003-4848-0501","position":4,"is_corresponding":false},{"id":63630,"name":"Yue Xiong","orcid":"0000-0003-2744-6566","position":5,"is_corresponding":false},{"id":397386,"name":"Yan Lin","orcid":"0000-0003-4956-861X","position":6,"is_corresponding":false},{"id":526983,"name":"Tengfei Zhang","orcid":"0000-0001-7556-0844","position":7,"is_corresponding":false},{"id":232660,"name":"Kun‐Liang Guan","orcid":"0000-0003-1892-0174","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:13:01.914939Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}