{"doi":"10.17615/a2xt-5g52","title":"The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3’-end processing","abstract":"Unlike canonical pre-mRNAs, animal replication-dependent histone pre-mRNAs lack introns and are processed at the 3’-end by a mechanism distinct from cleavage and polyadenylation. They have a 3’ stem loop and histone downstream element (HDE) that are recognized by stem-loop binding protein (SLBP) and U7 snRNP, respectively. The N-terminal domain (NTD) of Lsm11, a component of U7 snRNP, interacts with FLASH NTD and these two proteins recruit the histone cleavage complex containing the CPSF-73 endonuclease for the cleavage reaction. Here, we determined crystal structures of FLASH NTD and found that it forms a coiled-coil dimer. Using solution light scattering, we characterized the stoichiometry of the FLASH NTD-Lsm11 NTD complex and found that it is a 2:1 heterotrimer, which is supported by observations from analytical ultracentrifugation and crosslinking.","journal":"UNC Libraries","year":2020,"id":135858,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9515,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":274107,"name":"William F. Marzluff","orcid":"0000-0003-3635-4073","position":1,"is_corresponding":false},{"id":593377,"name":"Dazhi Tan","orcid":"0000-0002-8925-9347","position":2,"is_corresponding":false},{"id":593378,"name":"Chi‐Yuan Chou","orcid":"0000-0002-3400-171X","position":3,"is_corresponding":false},{"id":594109,"name":"Min-Han Lin","orcid":null,"position":4,"is_corresponding":false},{"id":280535,"name":"Ashutosh Tripathy","orcid":"0000-0001-9922-735X","position":5,"is_corresponding":false},{"id":274106,"name":"Wei Shen Aik","orcid":"0000-0003-4414-173X","position":6,"is_corresponding":false},{"id":274108,"name":"Zbigniew Domiński","orcid":"0000-0001-5100-7796","position":7,"is_corresponding":false},{"id":86983,"name":"Liang Tong","orcid":"0000-0002-0563-6468","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:35.237665Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}