{"doi":"10.17615/8pvm-dx28","title":"Longitudinal analysis of acute and convalescent B cell responses in a human primary dengue serotype 2 infection model","abstract":"Background: Acute viral infections induce a rapid and transient increase in antibody-secreting plasmablasts. At convalescence, memory B cells (MBC) and long-lived plasma cells (LLPC) are responsible for long-term humoral immunity. Following an acute viral infection, the specific properties and relationships between antibodies produced by these B cell compartments are poorly understood. Methods:Weutilized a controlled human challenge model of primary dengue virus serotype 2 (DENV2) infection to study acute and convalescent B-cell responses. Findings: The level of DENV2 replication was correlated with the magnitude of the plasmablast response. Functional analysis of plasmablast-derived monoclonal antibodies showed that the DENV2-specific response was dominated by cells producing DENV2 serotype-specific antibodies. DENV2-neutralizing antibodies targeted quaternary structure epitopes centered on domain III of the viral envelope protein (EDIII). Functional analysis ofMBC and serum antibodies from the same subjects six months post-challenge revealed maintenance of the serotypespecific response in both compartments. The serumresponse mainly targeted DENV2 serotype-specific epitopes on EDIII. Interpretation: Our data suggest overall functional alignment of DENV2-specific responses from the plasmablast, through the MBC and LLPC compartments following primary DENV2 inflection. These results provide enhanced resolution of the temporal and specificity of the B cell compartment in viral infection and serve as framework for evaluation of B cell responses in challenge models.","journal":"UNC Libraries","year":2020,"id":134998,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9527,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":5952,"name":"Ralph S. Baric","orcid":"0000-0001-6827-8701","position":1,"is_corresponding":false},{"id":246856,"name":"Sean A. Diehl","orcid":"0000-0001-9700-235X","position":2,"is_corresponding":false},{"id":591647,"name":"Bhumi Patel","orcid":null,"position":3,"is_corresponding":false},{"id":246860,"name":"Beth D. Kirkpatrick","orcid":"0000-0002-7016-7338","position":4,"is_corresponding":false},{"id":429329,"name":"Huy A. Tu","orcid":null,"position":5,"is_corresponding":false},{"id":428374,"name":"Usha K. Nivarthi","orcid":"0000-0002-7612-5649","position":6,"is_corresponding":false},{"id":573925,"name":"Daniel Emerling","orcid":"0000-0002-8754-9201","position":7,"is_corresponding":false},{"id":254644,"name":"Jesica Swanstrom","orcid":"0000-0002-5972-3719","position":8,"is_corresponding":false},{"id":342842,"name":"Stephen S. Whitehead","orcid":"0000-0001-6707-482X","position":9,"is_corresponding":false},{"id":16008,"name":"Ngan Luu-Thuy Nguyen","orcid":"0000-0003-3931-849X","position":10,"is_corresponding":false},{"id":342841,"name":"Anna P. Durbin","orcid":"0000-0002-6652-2851","position":11,"is_corresponding":false},{"id":338272,"name":"Kristen K. Pierce","orcid":null,"position":12,"is_corresponding":false},{"id":429330,"name":"Matthew J. Delacruz","orcid":null,"position":13,"is_corresponding":false},{"id":105519,"name":"Aravinda de Silva","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:16:27.963294Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}