{"doi":"10.17615/6e19-fq73","title":"Control of Meiotic Recombination and Gene Expression in Yeast by a Simple Repetitive DNA Sequence That Excludes Nucleosomes","abstract":"Tandem repeats of the pentanucleotide 5′-CCGNN (where N indicates any base) were previously shown to exclude nucleosomes in vitro (Y.-H. Wang and J. D. Griffith, Proc. Natl. Acad. Sci. USA 93:8863–8867, 1996). To determine the in vivo effects of these sequences, we replaced the upstream regulatory sequences of theHIS4gene ofSaccharomyces cerevisiaewith either 12 or 48 tandem copies of CCGNN. Both tracts activatedHIS4transcription. We found that (CCGNN)12tracts elevated meiotic recombination (hot spot activity), whereas the (CCGNN)48tract repressed recombination (cold spot activity). In addition, a “pure” tract of (CCGAT)12activated both transcription and meiotic recombination. We suggest that the cold spot activity of the (CCGNN)48tract is related to the phenomenon of the suppressive interactions of adjacent hot spots previously described in yeast (Q.-Q. Fan, F. Xu, and T. D. Petes, Mol. Cell. Biol. 15:1679–1688, 1995; Q.-Q. Fan, F. Xu, M. A. White, and T. D. Petes, Genetics 145:661–670, 1997; T.-C. Wu and M. Lichten, Genetics 140:55–66, 1995; L. Xu and N. Kleckner, EMBO J. 16:5115–5128, 1995).","journal":"UNC Libraries","year":2020,"id":144276,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.952,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":483061,"name":"Thomas D. Petes","orcid":"0000-0003-4890-8002","position":1,"is_corresponding":false},{"id":599604,"name":"Margaret Dominska","orcid":null,"position":2,"is_corresponding":false},{"id":613278,"name":"David T. Kirkpatrick","orcid":null,"position":3,"is_corresponding":false},{"id":307697,"name":"Jack D. Griffith","orcid":"0000-0002-8357-8689","position":4,"is_corresponding":false},{"id":422428,"name":"Yuh‐Hwa Wang","orcid":"0000-0002-9804-9368","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:45.339291Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}