{"doi":"10.17615/4n9w-q276","title":"DNA methylation of imprinted gene control regions in the regression of low-grade cervical lesions","abstract":"The role of host epigenetic mechanisms in the natural history of low-grade cervical intraepithelial neoplasia (CIN1) is not well characterized. We explored differential methylation of imprinted gene regulatory regions as predictors of the risk of CIN1 regression. A total of 164 patients with CIN1 were recruited from 10 Duke University clinics for the CIN Cohort Study. Participants had colposcopies at enrollment and up to five follow-up visits over 3 years. DNA was extracted from exfoliated cervical cells for methylation quantitation at CpG (cytosine-phosphate-guanine) sites and human papillomavirus (HPV) genotyping. Hazard ratios (HR) and 95% confidence intervals (CI) were estimated using Cox regression to quantify the effect of methylation on CIN1 regression over two consecutive visits, compared to non-regression (persistent CIN1; progression to CIN2+; or CIN1 regression at a single time-point), adjusting for age, race, high-risk HPV (hrHPV), parity, oral contraceptive and smoking status. Median participant age was 26.6 years (range: 21.0–64.4 years), 39% were African-American, and 11% were current smokers. Most participants were hrHPV-positive at enrollment (80.5%). Over one-third of cases regressed (n = 53, 35.1%). Median time-to-regression was 12.6 months (range: 4.5–24.0 months). Probability of CIN1 regression was negatively correlated with methylation at IGF2AS CpG 5 (HR = 0.41; 95% CI = 0.23–0.77) and PEG10 DMR (HR = 0.80; 95% CI = 0.65–0.98). Altered methylation of imprinted IGF2AS and PEG10 DMRs may play a role in the natural history of CIN1. If confirmed in larger studies, further research on imprinted gene DMR methylation is warranted to determine its efficacy as a biomarker for cervical cancer screening.","journal":"UNC Libraries","year":2024,"id":498872,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9646,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":270429,"name":"Susan K. Murphy","orcid":"0000-0001-8298-7272","position":1,"is_corresponding":false},{"id":33513,"name":"Rex C. Bentley","orcid":"0000-0002-4947-9150","position":2,"is_corresponding":false},{"id":474119,"name":"M.G. Hudgens","orcid":null,"position":3,"is_corresponding":false},{"id":594584,"name":"Fidel A. Valea","orcid":"0000-0002-4844-2399","position":4,"is_corresponding":false},{"id":526071,"name":"K.E. North","orcid":null,"position":5,"is_corresponding":false},{"id":696885,"name":"Ayodele Gomih","orcid":"0000-0002-9854-8888","position":6,"is_corresponding":false},{"id":1190797,"name":"R.L. Maguire","orcid":null,"position":7,"is_corresponding":false},{"id":412639,"name":"C. Hoyo","orcid":null,"position":8,"is_corresponding":false},{"id":1346427,"name":"RL Jirtle","orcid":null,"position":9,"is_corresponding":false},{"id":544132,"name":"David Skaar","orcid":"0000-0003-3530-957X","position":10,"is_corresponding":false},{"id":453040,"name":"Wendy R. Brewster","orcid":"0000-0001-6150-3160","position":11,"is_corresponding":false},{"id":345646,"name":"Zhiqing Huang","orcid":"0000-0001-5092-9297","position":12,"is_corresponding":false},{"id":453988,"name":"J.S. Smith","orcid":null,"position":13,"is_corresponding":false},{"id":325360,"name":"Adriana C. Vidal","orcid":"0000-0002-3240-6668","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:09:45.971055Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}