{"doi":"10.17615/4jmv-qf71","title":"Neutralizing the anticoagulant activity of ultra-low-molecular-weight heparins using N -acetylglucosamine 6-sulfatase","abstract":"Heparin has been the most commonly used anticoagulant drug for nearly a century. The drug heparin is generally categorized into three forms according to its molecular weight (MW), unfractionated (UF, average MW 13,000), low molecular weight (LMW, average MW 5,000), and ultra-low molecular weight heparin (ULMWH, average MW 2,000). Overdose of anticoagulant heparin can lead to very dangerous bleeding in patients. Protamine sulfate can be administered as an antidote to reverse heparin’s anticoagulant effect. There is not an effective antidote for ULMWH. In the current study, we examine human N-acetylglucosamine 6-sulfatase (NG6S), expressed in Chinese hamster ovary cells as a reversal agent for ULMWH. NG6S removes a single 6-O-sulfo group at the non-reducing end of the ULMWH Arixtra® (fondaparinux) effectively removing its ability to bind to antithrombin and preventing its inhibition of coagulation factor Xa. These results pave the way to develop human NG6S as an antidote for neutralizing the anticoagulant activity of ULMWHs.","journal":"UNC Libraries","year":2020,"id":141362,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9561,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":603591,"name":"Lingyun Li","orcid":"0000-0003-0822-7181","position":1,"is_corresponding":false},{"id":288986,"name":"Jian Liu","orcid":"0000-0001-8552-1400","position":2,"is_corresponding":false},{"id":592369,"name":"Xianxuan Zhou","orcid":"0000-0003-0105-5161","position":3,"is_corresponding":false},{"id":286697,"name":"Robert J. Linhardt","orcid":"0000-0003-2219-5833","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:24.429854Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}