{"doi":"10.17615/4akg-nr71","title":"A serine-substituted P450 catalyzes highly efficient carbene transfer to olefins in vivo","abstract":"Genetically encoded catalysts for non-natural chemical reactions will open new routes to sustainable production of chemicals. We designed a unique serine-heme ligated cytochrome “P411” that catalyzes efficient and selective carbene transfers from diazoesters to olefins in intact Escherichia coli cells. The mutation C400S in cytochrome P450BM3 gives a signature ferrous-CO Soret peak at 411 nm, abolishes monooxygenation activity, raises the resting state FeIII/II reduction potential, and significantly improves NAD(P)H-driven cyclopropanation activity.","journal":"UNC Libraries","year":2020,"id":144054,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9562,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":272467,"name":"Frances H. Arnold","orcid":"0000-0002-4027-364X","position":1,"is_corresponding":false},{"id":612573,"name":"Maraia E. Ener","orcid":null,"position":2,"is_corresponding":false},{"id":374770,"name":"Eric M. Brustad","orcid":"0000-0002-7031-5433","position":3,"is_corresponding":false},{"id":612574,"name":"Z Jane Wang","orcid":null,"position":4,"is_corresponding":false},{"id":612076,"name":"Stefanie A. Baril","orcid":"0000-0003-3249-2866","position":5,"is_corresponding":false},{"id":612575,"name":"Arvind Kannan","orcid":null,"position":6,"is_corresponding":false},{"id":612075,"name":"Pedro S. Coelho","orcid":"0000-0003-0828-9956","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:41.821196Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}