{"doi":"10.17615/40vp-8m98","title":"HIV-Specific T Cells Generated from Naive T Cells Suppress HIV In Vitro and Recognize Wide Epitope Breadths","abstract":"The Berlin Patient represents the first and only functional HIV cure achieved by hematopoietic stem cell transplant (HSCT). In subsequent efforts to replicate this result, HIV rebounded post-HSCT after withdrawal of antiretroviral therapy. Providing HIV-specific immunity through adoptive T cell therapy may prevent HIV rebound post-HSCT by eliminating newly infected cells before they can seed systemic infection. Adoptive T cell therapy has demonstrated success in boosting Epstein-Barr virus and cytomegalovirus-specific immunity post-HSCT, controlling viral reactivation. However, T cell immunotherapies to boost HIV-specific immunity have been limited by single-epitope specificity and minimal persistence or efficacy in vivo. To improve this strategy, we sought to generate allogeneic HIV-specific T cells from human leukocyte antigen (HLA)-A02+ HIV-negative adult or cord blood donors. We focused on HLA-A02+ donors due to well-characterized epitope restrictions observed in HIV+ populations. We show that multi-antigen HIV-specific T cells can be generated from naive T cells of both cord blood and adults using a reproducible good manufacturing practice (GMP)-grade protocol. This product lysed antigen-pulsed targets and suppressed active HIV in vitro. Interestingly, these cells displayed broad epitope recognition despite lacking recognition of the common HLA-A02-restricted HIV epitope Gag SL9. This first demonstration of functional multi-antigen HIV-specific T cells has implications for improving treatment of HIV through allogeneic HSCT. Patel et al. demonstrate the ability to generate HIV-specific T cells from HIV-seronegative adults and cord blood with a good-manufacturing-practice-compliant strategy. These immunotherapies are multi-antigen specific, display cytotoxicity, and suppress HIV in vitro, providing a promising platform for adoptive T cell therapy in a post-transplant setting.","journal":"UNC Libraries","year":2020,"id":140608,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9511,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":533804,"name":"N. Archin","orcid":null,"position":1,"is_corresponding":false},{"id":549390,"name":"J.J. Eron","orcid":null,"position":2,"is_corresponding":false},{"id":605429,"name":"C.M. Rooney","orcid":null,"position":3,"is_corresponding":false},{"id":590985,"name":"C.L. Gay","orcid":null,"position":4,"is_corresponding":false},{"id":604810,"name":"J.A. Sung","orcid":"0009-0003-3142-3647","position":5,"is_corresponding":false},{"id":398654,"name":"S. Patel","orcid":null,"position":6,"is_corresponding":false},{"id":605430,"name":"L. Roesch","orcid":null,"position":7,"is_corresponding":false},{"id":605431,"name":"T. Tripic","orcid":null,"position":8,"is_corresponding":false},{"id":605432,"name":"C.R. Cruz","orcid":null,"position":9,"is_corresponding":false},{"id":534476,"name":"D.M. Margolis","orcid":null,"position":10,"is_corresponding":false},{"id":590982,"name":"J.D. Kuruc","orcid":null,"position":11,"is_corresponding":false},{"id":605433,"name":"C.M. Bollard","orcid":null,"position":12,"is_corresponding":false},{"id":590988,"name":"N. Goonetilleke","orcid":null,"position":13,"is_corresponding":false},{"id":605434,"name":"M.L. Clohosey","orcid":null,"position":14,"is_corresponding":false},{"id":605428,"name":"P.J. Hanley","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:17.077903Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}