{"doi":"10.17615/3sjj-eq92","title":"mAM Facilitates Conversion by ESET of Dimethyl to Trimethyl Lysine 9 of Histone H3 to Cause Transcriptional Repression","abstract":"Methylation of histone tails plays an important role in chromatin structure and function. Previously, we reported that ESET/SETDB1 is a histone methyltransferase (HMTase). Here, we show that SETDB1 tightly associates with the human homolog of mAM, a murine ATFa-associated factor. Although recombinant ESET can methylate lysine 9 of histone H3 (H3-K9), its activity is severely compromised when compared to that of the ESET/mAM complex. mAM stimulates ESET enzymatic activity by increasing the Vmax and decreasing the Km. Importantly, mAM facilitates the ESET-dependent conversion of dimethyl H3-K9 to the trimethyl state both in vitro and in vivo. Chromatin-based transcription and ChIP analyses demonstrate that mAM enhances ESET-mediated transcriptional repression in a SAM-dependent manner, and this repression correlates with H3-K9 trimethylation at the promoter. Thus, our studies establish that promoter H3-K9 trimethylation is the cause of transcriptional repression and that mAM/hAM facilitates conversion of H3-K9 dimethyl to trimethyl by ESET/SETDB1.","journal":"UNC Libraries","year":2021,"id":205605,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":18,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9542,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2021-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":246358,"name":"Robert G. Roeder","orcid":"0000-0003-3865-8572","position":1,"is_corresponding":false},{"id":38288,"name":"Paul Tempst","orcid":"0000-0002-6680-3987","position":2,"is_corresponding":false},{"id":495578,"name":"Hengbin Wang","orcid":"0000-0003-2414-0609","position":3,"is_corresponding":false},{"id":788899,"name":"Bruno Chatton","orcid":"0000-0003-4515-7119","position":4,"is_corresponding":false},{"id":788900,"name":"Yi Zhang","orcid":"0000-0003-4769-6642","position":5,"is_corresponding":false},{"id":542876,"name":"Woojin An","orcid":"0000-0001-8756-595X","position":6,"is_corresponding":false},{"id":439225,"name":"Xia Li","orcid":"0000-0002-6068-5182","position":7,"is_corresponding":false},{"id":291559,"name":"Hediye Erdjument‐Bromage","orcid":"0000-0003-0224-3594","position":8,"is_corresponding":false},{"id":528925,"name":"Ru Cao","orcid":"0000-0003-3776-8008","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:51:33.996102Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}