{"doi":"10.17615/2s0y-wp97","title":"Pannexin 1 channels mediate ‘find-me’ signal release and membrane permeability during apoptosis","abstract":"Apoptotic cells release ‘find-me’ signals at the earliest stages of death to recruit phagocytes1. The nucleotides ATP and UTP represent one class of find-me signals2, but their mechanism of release is not known. Here, we identify the plasma membrane channel pannexin 1 (PANX1) as a mediator of find-me signal/nucleotide release from apoptotic cells. Pharmacological inhibition and siRNA-mediated knockdown of PANX1 led to decreased nucleotide release and monocyte recruitment by apoptotic cells. Conversely, PANX1 over-expression enhanced nucleotide release from apoptotic cells and phagocyte recruitment. Patch-clamp recordings showed that PANX1 was basally inactive, and that induction of PANX1 currents occurred only during apoptosis. Mechanistically, PANX1 itself was a target of effector caspases (caspases 3 and 7), and a specific caspase-cleavage site within PANX1 was essential for PANX1 function during apoptosis. Expression of truncated PANX1 (at the putative caspase cleavage site) resulted in a constitutively open channel. PANX1 was also important for the ‘selective’ plasma membrane permeability of early apoptotic cells to specific dyes3. Collectively, these data identify PANX1 as a plasma membrane channel mediating the regulated release of find-me signals and selective plasma membrane permeability during apoptosis, and a new mechanism of PANX1 activation by caspases.","journal":"UNC Libraries","year":2020,"id":139361,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9527,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":602142,"name":"Douglas A. Bayliss","orcid":"0000-0002-5630-2572","position":1,"is_corresponding":false},{"id":90292,"name":"Guy S. Salvesen","orcid":"0000-0002-7933-6732","position":2,"is_corresponding":false},{"id":602411,"name":"Faraaz B. Chekeni","orcid":null,"position":3,"is_corresponding":false},{"id":481860,"name":"Allison J. Armstrong","orcid":null,"position":4,"is_corresponding":false},{"id":593479,"name":"Dale W. Laird","orcid":"0000-0002-4568-3285","position":5,"is_corresponding":false},{"id":260505,"name":"Kodi S. Ravichandran","orcid":"0000-0001-9049-1410","position":6,"is_corresponding":false},{"id":600910,"name":"Silvia Peñuela","orcid":"0000-0003-4829-5517","position":7,"is_corresponding":false},{"id":602409,"name":"Scott F. Walk","orcid":null,"position":8,"is_corresponding":false},{"id":342340,"name":"Michael R. Elliott","orcid":"0000-0003-4128-5527","position":9,"is_corresponding":false},{"id":602757,"name":"Joanna K. Sandilos","orcid":null,"position":10,"is_corresponding":false},{"id":279524,"name":"Eduardo R. Lazarowski","orcid":"0000-0002-5419-351X","position":11,"is_corresponding":false},{"id":190934,"name":"Brant E. Isakson","orcid":"0000-0002-7692-6294","position":12,"is_corresponding":false},{"id":339084,"name":"Jason M. Kinchen","orcid":"0000-0001-8430-2265","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:10.185988Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}