{"doi":"10.17615/1kpj-1p06","title":"Tracking replication enzymology in vivo by genome-wide mapping of ribonucleotide incorporation","abstract":"Ribonucleotides are frequently incorporated into DNA during eukaryotic replication. Here we map the genome-wide distribution of these ribonucleotides as markers of replication enzymology in budding yeast, using a new 5′-DNA end-mapping method, Hydrolytic End Sequencing. HydEn-Seq of DNA from ribonucleotide excision repair-deficient strains reveals replicase- and strand-specific patterns of ribonucleotides in the nuclear genome. These patterns support the role of DNA polymerases α and δ in lagging strand replication and of DNA polymerase ε in leading strand replication. They identify replication origins, termination zones and variations in ribonucleotide incorporation frequency across the genome that exceed three orders of magnitude. HydEn-Seq also reveals strand-specific 5′-DNA ends at mitochondrial replication origins, suggesting unidirectional replication of a circular genome. Given the conservation of enzymes that incorporate and process ribonucleotides in DNA, HydEn-Seq can be used to track replication enzymology in other organisms.","journal":"UNC Libraries","year":2020,"id":138037,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9476,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":63126,"name":"David C. Fargo","orcid":"0000-0003-4728-2642","position":1,"is_corresponding":false},{"id":280462,"name":"Adam Burkholder","orcid":"0000-0002-0237-6920","position":2,"is_corresponding":false},{"id":280461,"name":"Scott A. Lujan","orcid":"0000-0003-2400-8325","position":3,"is_corresponding":false},{"id":13808,"name":"Piotr A. Mieczkowski","orcid":"0000-0003-2418-0096","position":4,"is_corresponding":false},{"id":505270,"name":"Duncan J. Smith","orcid":"0000-0002-0898-8629","position":5,"is_corresponding":false},{"id":599455,"name":"Maryam Clausen","orcid":null,"position":6,"is_corresponding":false},{"id":108245,"name":"Ewa P. Malc","orcid":"0000-0002-4596-1142","position":7,"is_corresponding":false},{"id":73052,"name":"Thomas A. Kunkel","orcid":"0000-0002-9900-1788","position":8,"is_corresponding":false},{"id":599456,"name":"Clinton D. Orebaugh","orcid":null,"position":9,"is_corresponding":false},{"id":598764,"name":"Jessica S. Williams","orcid":"0000-0002-9758-5812","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":{"citation_network_status":"fetched"},"created_at":"2026-07-18T23:16:59.449905Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}