{"doi":"10.17615/15aw-5970","title":"A Sterol-Binding Protein Integrates Endosomal Lipid Metabolism with TOR Signaling and Nitrogen Sensing","abstract":"Kes1, and other oxysterol binding protein (OSBP) superfamily members, are involved in membrane and lipid trafficking through trans-Golgi network (TGN) and endosomal systems. We demonstrate that Kes1 represents a sterol-regulated antagonist of TGN/endosomal phosphatidylinositol-4-phosphate signaling. This regulation modulates TOR activation by amino acids, and dampens gene expression driven by Gcn4; the primary transcriptional activator of the general amino acid control regulon. Kes1-mediated repression of Gcn4 transcription factor activity is characterized by nonproductive Gcn4 binding to its target sequences, involves TGN/endosome-derived sphingolipid signaling, and requires activity of the cyclin-dependent kinase 8 (CDK8) module of the enigmatic ‘large Mediator’ complex. These data describe a pathway by which Kes1 integrates lipid metabolism with TORC1 signaling and nitrogen sensing.","journal":"UNC Libraries","year":2020,"id":143336,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9548,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":366112,"name":"Peihua Yuan","orcid":"0000-0002-4633-0692","position":1,"is_corresponding":false},{"id":610965,"name":"Paul K. Herman","orcid":"0000-0002-2051-8355","position":2,"is_corresponding":false},{"id":540410,"name":"Brian J. Dewar","orcid":null,"position":3,"is_corresponding":false},{"id":380707,"name":"Naseem A. Gaur","orcid":"0000-0002-1224-8789","position":4,"is_corresponding":false},{"id":610966,"name":"Max Wolpert","orcid":"0000-0001-5312-710X","position":5,"is_corresponding":false},{"id":539433,"name":"Carl J. Mousley","orcid":"0000-0002-0192-7030","position":6,"is_corresponding":false},{"id":430133,"name":"Aaron H. Nile","orcid":"0000-0002-7370-8769","position":7,"is_corresponding":false},{"id":611417,"name":"Stephen J. Deminoff","orcid":null,"position":8,"is_corresponding":false},{"id":366120,"name":"Vytas A. Bankaitis","orcid":"0000-0002-1654-6759","position":9,"is_corresponding":false},{"id":592619,"name":"Kyle Trettin","orcid":null,"position":10,"is_corresponding":false},{"id":524572,"name":"Jeffrey M. Macdonald","orcid":null,"position":11,"is_corresponding":false},{"id":326039,"name":"Alan G. Hinnebusch","orcid":"0000-0002-1627-8395","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:17:38.432803Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}