{"doi":"10.17615/0yqc-bf83","title":"Cryo-EM structure of an antibody that neutralizes dengue virus type 2 by locking E protein dimers","abstract":"There are four closely-related dengue virus (DENV) serotypes. Infection with one serotype generates antibodies that may cross-react and enhance infection with other serotypes in a secondary infection. We demonstrated that DENV serotype 2 (DENV2)–specific human monoclonal antibody (HMAb) 2D22 is therapeutic in a mouse model of antibody-enhanced severe dengue disease. We determined the cryo–electron microscopy (cryo-EM) structures of HMAb 2D22 complexed with two different DENV2 strains. HMAb 2D22 binds across viral envelope (E) proteins in the dimeric structure, which probably blocks the E protein reorganization required for virus fusion. HMAb 2D22 “locks” two-thirds of or all dimers on the virus surface, depending on the strain, but neutralizes these DENV2 strains with equal potency. The epitope defined by HMAb 2D22 is a potential target for vaccines and therapeutics.","journal":"UNC Libraries","year":2020,"id":115450,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":3,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9562,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2020-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":542023,"name":"Jr. Crowe","orcid":null,"position":1,"is_corresponding":false},{"id":393812,"name":"A. M. de Silva","orcid":null,"position":2,"is_corresponding":false},{"id":515736,"name":"E. Harris","orcid":null,"position":3,"is_corresponding":false},{"id":542024,"name":"V. A. Kostyuchenko","orcid":null,"position":4,"is_corresponding":false},{"id":542025,"name":"J. S. G. Ooi","orcid":null,"position":5,"is_corresponding":false},{"id":542026,"name":"X.-N. Lim","orcid":null,"position":6,"is_corresponding":false},{"id":542027,"name":"Jinghan Tan","orcid":null,"position":7,"is_corresponding":false},{"id":515738,"name":"S. A. Smith","orcid":null,"position":8,"is_corresponding":false},{"id":515741,"name":"K. D. Ibarra","orcid":null,"position":9,"is_corresponding":false},{"id":542028,"name":"T.-S. Ng","orcid":null,"position":10,"is_corresponding":false},{"id":303768,"name":"G. Fibriansah","orcid":"0000-0002-9990-3335","position":11,"is_corresponding":false},{"id":542029,"name":"S.-M. Lok","orcid":null,"position":12,"is_corresponding":false},{"id":453009,"name":"J. Wang","orcid":"0009-0004-9223-8493","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-18T23:13:36.820928Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}