{"doi":"10.17504/protocols.io.x54v9be5ml3e/v1","title":"Accurate detection of somatic mutations in single cells by scNanoSeq v1","abstract":"Accurately detecting somatic mutations in individual human cells poses a significant challenge for single-cell whole genome amplification methods. In this study, we introduce a novel single-cell genome amplification method based on the restriction enzyme-based NanoSeq chemistry, achieving an error rate of less than 10-10in mutation calling (equivalent to 0.2 errors per human genome). We term this methodology as scNanoSeq. This precision is derived from theoretical error rate analyses within the duplexing sequencing scheme, which aligns with upper bound estimations derived from single-cell expansion experiments.","journal":null,"year":2025,"id":586627,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9494,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2025-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":237547,"name":"Yang Zhang","orcid":"0000-0001-7462-2935","position":1,"is_corresponding":false},{"id":69159,"name":"Jiayi Luo","orcid":null,"position":2,"is_corresponding":false},{"id":68992,"name":"Chenghang Zong","orcid":"0000-0002-8337-8038","position":3,"is_corresponding":false},{"id":621383,"name":"Muchun Niu","orcid":"0000-0003-3221-3695","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:59:32.191237Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}