{"doi":"10.17504/protocols.io.rm7vzy4e4lx1/v1","title":"Pre-Extraction and Matrix Application via a HTX M5 Sprayer for Intact Proteoform MALDI Imaging v1","abstract":"Scope: Using an HTX M5 sprayer this protocol outlines a pre-extraction step for increased sensitivity for intact proteoform analyses, this is an optional step within the MALDI-MSI pipeline for intact proteoform mapping using a Spectroglyph EP-MALDI-2 source coupled to a custom UHMR HF Orbitrap. This is followed by the coating and optional recrystallization of MALDI matrix on the sample, all steps have been completed on both human kidney and human pancreas. Other tissue types should go through validation. Expected Outcomes: A tissue section prepared for intact proteoform analyses.","journal":null,"year":2022,"id":310672,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9541,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2022-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":443408,"name":"Kevin Zemaitis","orcid":"0000-0002-3524-9776","position":0,"is_corresponding":true}],"reference_count":1,"raw_metadata":null,"created_at":"2026-07-19T00:33:24.451129Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}