{"doi":"10.17504/protocols.io.n92ld8o79v5b/v1","title":"Zika virus NS2B-NS3 protease binding assay for compound screen using grating-coupled interferometry v1","abstract":"This protocol aims to measure kinetic parameters (ka, kd, and KD) for compounds against Zika virus NS2B-NS3 protease using the Creoptix Wave system. Grating coupled interferometry (GCI) is a label-free technique to measure kinetics and affinity for different targets (proteins, small molecules, fragments, etc.) with enhanced sensitivity. The stable attachment of His-tagged NS2B-NS3 protease to the chip surface was achieved through His capture and amine coupling strategy. This immobilization technique provides oriented and active protein immobilization for binding assays and can be applied to different protein targets. Binding analysis was performed with the Repeated Analyte Pulses of Increasing Duration (RAPID) method, which involves the injection of samples at a single concentration with varied association times. Kinetic parameters were obtained from Creoptix Wave software (v 4.5.18).","journal":null,"year":2024,"id":508391,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9666,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1339556,"name":"Korvus Wang","orcid":null,"position":1,"is_corresponding":false},{"id":1329719,"name":"Xiaomin Ni","orcid":"0000-0002-7769-8297","position":2,"is_corresponding":false},{"id":1003047,"name":"L. Koekemoer","orcid":"0000-0001-9226-9127","position":3,"is_corresponding":false},{"id":1360503,"name":"warren.thompson not provided","orcid":null,"position":4,"is_corresponding":false},{"id":552166,"name":"F. von Delft","orcid":"0000-0003-0378-0017","position":5,"is_corresponding":false},{"id":1329722,"name":"Mary-Ann Xavier","orcid":"0000-0001-9672-0763","position":6,"is_corresponding":false},{"id":1329717,"name":"Eda Çapkın","orcid":"0000-0003-4570-6683","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:11:10.049781Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}