{"doi":"10.17504/protocols.io.n92ld897ov5b/v1","title":"Master_Setup and Operation of Single Use (SUB) Bubble Column Reactors (BCR): Litre-Scale Expression of Recombinant Proteins for Structural Biology and Drug Design (SBDD). v1","abstract":"Structure-Based Drug Discovery (SBDD) is an approach to drug design which involves crystallising disease target proteins unbound and with candidate small-molecule binders, to observe and analyse the binding interaction, or to identify novel binders that can be developed into lead-like compounds. Obtaining sufficient protein quantity and crystal forms amenable to soaking with candidate binders remains a bottleneck. The Centre for Medicines Discovery’s (CMD) approach to this problem, is to design multiple constructs to explore protein expression and crystallisation behaviour. Protein crystallography (PX) has a high demand for protein quantities, a limiting factor in this workflow is the production of sufficient quantities of the multiple proteins for our experimental needs. This burden increases rapidly with the addition of each construct. Existing shake-flask workflows for protein expression do not scale adequately to meet these requirements. To address this bottleneck a novel, single-use Bubble-Column Bioreactor (suBCR) array was developed, that allows for parallel 1 L E. coli batch cultivation. This bioreactor array will be incorporated into a Gene-to-Product workflow aimed at increasing protein production efficiency by increasing throughput whilst reducing labour burden.","journal":null,"year":2024,"id":508497,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9453,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1090393,"name":"Nathan T. Wright","orcid":"0000-0003-0177-6129","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:11:10.049781Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}