{"doi":"10.17504/protocols.io.8epv5rm96g1b/v1","title":"iPSC Pneumocyte Live Virus SARS-CoV-2 Cytotoxicity Screening Assay v1","abstract":"These immunofluorescence-based antiviral assays rely on identification of infected cells through immunostaining the NP (influenza virus), N (SARS-CoV-2) proteins, or any relevant viral antigen at 488nM and counterstaining with DAPI. Infected cells (488nM) and total cells (DAPI) are identified andquantified by a plate cytometer (Biotek Cytation 1). Compounds are tested in a triplicate 8-pointdose-response with concurrent cytotoxicity assays (MTT Roche/MTS Promega) in uninfectedcells. These assays can be run on any adherent cell type, such as standard cell lines and iPSC-derived cell types. DMSO infection controls (negative) and uninfected cell controls (positive) should be included on every plate. The DMSO controls will establish 100% infection and the uninfected wells 0% infection for each plate. Relevant control compounds should be included as controls for every run. The effect p-gp inhibitors on known p-gp subtrates should be explored in all new cell types used. These assays accurately reflect the antiviral activity of compounds in medium-to-high throughput, depending on the level of automation.","journal":null,"year":2024,"id":508562,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9491,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1481,"name":"Kris M. White","orcid":"0000-0003-0889-0506","position":1,"is_corresponding":false},{"id":1478,"name":"Briana L. McGovern","orcid":"0000-0002-0492-9904","position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:11:10.049781Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}