{"doi":"10.17504/protocols.io.8epv5rd5dg1b/v1","title":"Cell sorting (FACS) for viable cells, non-neuronal and neuronal cells from mice, primates and humans v1","abstract":"Cell sorting is a key step for many multi-step procedures defining cell and gene plasticity, such as single-cell RNA sequencing, bulk RNA sequencing from specific type of cells, RT-PCR from specific population of cells, cell culturing and electrophysiology. This protocol outlines the steps for cell sorting (FACS) for live cells, for specific non-neuronal cell types and for specific types of sensory neurons. Accordingly, this protocol outlines the detailed steps for preparing and sorting live cells using Calcein Violet-AM and Helix NP NIR dyes, followed by cell sorting. The procedure includes the preparation of working solutions, cell suspension, labeling, and sorting of viable cells. Calcein Violet-AM is used to label live cells, while Helix NP NIR is utilized to exclude dead cells, ensuring high viability and purity of sorted cells. The protocol was adapted from established methodologies and our multiple studies, including Mecklenburg et al., Front Pain Res (Lausanne)(2023); Hovhannisyan et al., Scientific Reports (2021); and Mecklenburg et al., Scientific Reports(2020). Specifically, unlike the original studies, this protocol is validated for primate and human cells.","journal":null,"year":2024,"id":508696,"datarank":0.0,"base_score":0.0,"endowment":0.0,"self_citation_contribution":0.0,"citation_network_contribution":0.0,"self_endowment_contribution":0.0,"citer_contribution":0.0,"corpus_percentile":null,"corpus_rank":null,"citation_count":0,"citer_count":0,"citers_with_citation_signal":0,"citers_with_endowment":0,"datacite_reuse_total":0,"is_dataset":false,"is_dataset_confidence":0.9405,"is_data_producer":false,"deposit_databanks":null,"is_oa":true,"file_count":0,"downloads":0,"has_version_chain":false,"published_date":"2024-01-01","fair_score":null,"fair_percentile":null,"algorithm_id":"datarank_citation_only_1hop_v6","ranking_scope":"data_only","authors":[{"id":1360717,"name":"Sergey Shein","orcid":null,"position":1,"is_corresponding":false},{"id":1360716,"name":"Alexei Tumanov","orcid":null,"position":2,"is_corresponding":false},{"id":1360715,"name":"Armen Akopian","orcid":null,"position":3,"is_corresponding":false},{"id":290300,"name":"Jennifer Mecklenburg","orcid":null,"position":0,"is_corresponding":true}],"reference_count":0,"raw_metadata":null,"created_at":"2026-07-19T02:11:11.495781Z","pmid":null,"pmcid":null,"fwci":null,"citation_percentile":null,"influential_citations":0,"oa_status":null,"license":null,"views":0,"total_file_size_bytes":0,"version_count":0,"fair_f":null,"fair_a":null,"fair_i":null,"fair_r":null,"fair_zscore":null,"fair_rationale":null,"fair_model":null,"fair_agent_version":null,"fair_fulltext_source":null,"fair_has_llm":null,"fair_computed_at":null,"clinical_trials":[],"software_tools":[],"db_accessions":[],"linked_datasets":[],"topics":[]}